Detailed information of TA system TA127257

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 78695..79296
• Replicon: plasmid p853EC1
• Accession: NZ_CP040920
• Organism: Escherichia coli strain FC853_EC

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9YA20
• Locus tag: FH485_RS26005
• Protein ID: WP_001216045.1
• Coordinates: 78695..79075 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: FH485_RS26010
• Protein ID: WP_001190712.1
• Coordinates: 79075..79296 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH485_RS25980	74136..75620	-	1485	WP_000124155.1	hypothetical protein	-
FH485_RS25985	75620..76812	-	1193	Protein_69	terminase	-
FH485_RS25990	76899..77351	-	453	WP_001369802.1	hypothetical protein	-
FH485_RS25995	77440..78483	-	1044	WP_000648825.1	DUF968 domain-containing protein	-
FH485_RS26000	78511..78690	-	180	WP_000113019.1	hypothetical protein	-
FH485_RS26005	78695..79075	-	381	WP_001216045.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
FH485_RS26010	79075..79296	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FH485_RS26015	79369..79758	-	390	WP_000506726.1	DNA repair protein	-
FH485_RS26020	79882..80133	-	252	WP_001377386.1	DNA polymerase III subunit theta	-
FH485_RS28005	80583..80720	+	138	WP_000123562.1	hypothetical protein	-
FH485_RS26030	80795..81157	-	363	WP_001261544.1	hypothetical protein	-
FH485_RS26035	81154..82086	-	933	WP_077780118.1	hypothetical protein	-
FH485_RS26040	82068..82441	-	374	Protein_80	hypothetical protein	-
FH485_RS26045	82448..82741	-	294	WP_001677496.1	hypothetical protein	-
FH485_RS26050	82920..83153	-	234	WP_000516537.1	hypothetical protein	-
FH485_RS26060	83236..84120	-	885	WP_023356290.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..97266	97266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13588.29 Da
• Isoelectric Point: 5.1514

>T127257 WP_001216045.1 NZ_CP040920:c79075-78695 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T127257 NZ_CP040920:c79075-78695 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT127257 WP_001190712.1 NZ_CP040920:c79296-79075 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT127257 NZ_CP040920:c79296-79075 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A829CLP7

Antitoxin

• AlphaFold DB: A0A829CJB6