Detailed information of TA system TA127255

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PanAT/DUF4065(antitoxin)
• Location: 4590085..4591073
• Replicon: chromosome
• Accession: NZ_CP040919
• Organism: Escherichia coli strain FC853_EC

Toxin (Protein)

• Gene name: panT
• Uniprot ID: -
• Locus tag: FH485_RS22670
• Protein ID: WP_014640052.1
• Coordinates: 4590085..4590471 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: panA
• Uniprot ID: A0A0A1AFM1
• Locus tag: FH485_RS22675
• Protein ID: WP_000458583.1
• Coordinates: 4590501..4591073 (-)
• Length: 191 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH485_RS22625	4585227..4585424	-	198	WP_001231956.1	Cro/Cl family transcriptional regulator	-
FH485_RS22630	4585520..4586173	+	654	WP_000981537.1	helix-turn-helix domain-containing protein	-
FH485_RS22640	4586600..4586845	-	246	WP_000141753.1	hypothetical protein	-
FH485_RS22645	4586958..4587268	+	311	Protein_4346	hypothetical protein	-
FH485_RS22650	4587283..4587645	+	363	WP_000135680.1	hypothetical protein	-
FH485_RS22655	4587711..4588535	+	825	WP_023908886.1	DUF2303 family protein	-
FH485_RS22660	4588754..4589506	+	753	WP_024946495.1	hypothetical protein	-
FH485_RS22665	4589543..4589824	+	282	WP_001093916.1	pyocin activator PrtN family protein	-
FH485_RS27975	4589872..4590045	-	174	WP_023908888.1	hypothetical protein	-
FH485_RS22670	4590085..4590471	-	387	WP_014640052.1	hypothetical protein	Toxin
FH485_RS22675	4590501..4591073	-	573	WP_000458583.1	SocA family protein	Antitoxin
FH485_RS22680	4591309..4591431	-	123	WP_001300034.1	hypothetical protein	-
FH485_RS22685	4591793..4592797	-	1005	WP_022646425.1	DUF2713 family protein	-
FH485_RS22690	4593115..4593630	+	516	WP_000416263.1	zinc uptake transcriptional repressor Zur	-
FH485_RS22695	4593672..4593881	-	210	WP_001296638.1	CsbD family protein	-
FH485_RS22700	4593997..4595322	-	1326	WP_001361471.1	MATE family efflux transporter DinF	-
FH485_RS22705	4595395..4596003	-	609	WP_000646078.1	transcriptional repressor LexA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4555300..4596481	41181

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Antitoxin SocA-like, Panacea domain (27-137)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14465.40 Da
• Isoelectric Point: 5.4833

>T127255 WP_014640052.1 NZ_CP040919:c4590471-4590085 [Escherichia coli]
VLAPTGPCNHLHVICNDPVYYPVNDCYCVLVVNISSIKDGVPHDPSCVLNSGDHRFIKHPSYVVYAEAIIWRVDNMVRKQ
RSGEISVHDDMPEATFNRILDGFDISDEVTPKNLKFKNKYCVSSIDDE

Nucleotide

• Length: 387 bp

>T127255 NZ_CP040919:c4590471-4590085 [Escherichia coli]
GTGCTTGCCCCAACTGGGCCATGCAATCATCTTCATGTGATTTGTAATGATCCTGTTTATTACCCCGTTAACGATTGTTA
TTGTGTTTTAGTTGTTAATATTTCTAGTATCAAGGATGGTGTCCCCCACGATCCGTCTTGCGTCTTGAATTCTGGTGATC
ATCGCTTTATCAAGCATCCAAGTTATGTTGTTTACGCTGAAGCTATAATTTGGCGAGTGGATAACATGGTTAGAAAGCAG
CGATCGGGTGAGATTTCTGTTCATGATGATATGCCAGAAGCTACATTCAATAGAATTCTGGACGGTTTTGATATATCTGA
TGAAGTTACGCCAAAGAACCTTAAATTTAAAAATAAATATTGCGTATCATCTATTGATGATGAGTAA

Antitoxin

Protein

• Length: 191 a.a.
• Molecular weight: 21956.30 Da
• Isoelectric Point: 6.0283

>AT127255 WP_000458583.1 NZ_CP040919:c4591073-4590501 [Escherichia coli]
MFCEEKVAQMAAYLLLKRGGRMAYLKLMKLLYLSNRKSILKHGRMIGEDSLYSMKFGPVMSNTLNLIRGKAEGIGDYWYN
LIETNGHDVLLRSDPREMDADEVFDELSRADIRILDEIYSLYGHMNRFDLANMTHLESVCPEWHNPGNSRKPIDLKEMLI
SEGKSEDEANRIIGKMEESQKLKEFSLQLS

Nucleotide

• Length: 573 bp

>AT127255 NZ_CP040919:c4591073-4590501 [Escherichia coli]
ATGTTTTGCGAAGAAAAAGTAGCTCAAATGGCTGCATACCTACTTCTTAAGCGAGGTGGGCGCATGGCATATCTGAAATT
GATGAAGCTGCTCTATCTGTCTAACCGCAAGTCGATTTTGAAGCATGGCAGGATGATCGGCGAAGATAGTCTTTACTCTA
TGAAATTTGGTCCAGTCATGTCGAATACGCTGAACTTGATTCGTGGTAAGGCTGAAGGCATTGGTGACTACTGGTACAAC
TTGATAGAGACGAACGGGCATGATGTGTTGTTGCGTTCAGATCCGAGGGAAATGGATGCAGACGAGGTCTTTGATGAGTT
GAGCCGTGCAGATATCCGGATTTTAGATGAAATCTATTCTCTGTATGGGCATATGAACCGATTTGATCTCGCAAACATGA
CGCATTTAGAAAGCGTTTGTCCAGAGTGGCATAATCCTGGCAATTCTCGTAAGCCTATAGACCTGAAAGAAATGCTGATC
AGTGAGGGTAAAAGCGAGGATGAGGCTAATCGTATAATTGGCAAAATGGAAGAATCTCAGAAACTTAAGGAATTTTCTTT
GCAATTATCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0A1AFM1