Detailed information of TA system TA127244

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3226094..3226799
• Replicon: chromosome
• Accession: NZ_CP040919
• Organism: Escherichia coli strain FC853_EC

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: FH485_RS15870
• Protein ID: WP_000539521.1
• Coordinates: 3226094..3226480 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: FH485_RS15875
• Protein ID: WP_001280945.1
• Coordinates: 3226470..3226799 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH485_RS15850	3222098..3222724	+	627	WP_001295292.1	glutathione S-transferase GstB	-
FH485_RS15855	3222721..3223836	-	1116	WP_000554964.1	aldose sugar dehydrogenase YliI	-
FH485_RS15860	3223947..3224330	-	384	WP_000497137.1	biofilm formation regulator BssR	-
FH485_RS15865	3224543..3225868	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
FH485_RS15870	3226094..3226480	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FH485_RS15875	3226470..3226799	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
FH485_RS15880	3226869..3228197	-	1329	WP_022645414.1	GGDEF domain-containing protein	-
FH485_RS15885	3228205..3230553	-	2349	WP_140028021.1	EAL domain-containing protein	-
FH485_RS15890	3230730..3231641	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T127244 WP_000539521.1 NZ_CP040919:3226094-3226480 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T127244 NZ_CP040919:3226094-3226480 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGTAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT127244 WP_001280945.1 NZ_CP040919:3226470-3226799 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT127244 NZ_CP040919:3226470-3226799 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6