Detailed information of TA system TA127242

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2535874..2536512
• Replicon: chromosome
• Accession: NZ_CP040919
• Organism: Escherichia coli strain FC853_EC

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: FH485_RS12425
• Protein ID: WP_000813794.1
• Coordinates: 2536336..2536512 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: FH485_RS12420
• Protein ID: WP_001270286.1
• Coordinates: 2535874..2536290 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH485_RS12400	2531026..2531967	-	942	WP_022645667.1	ABC transporter permease	-
FH485_RS12405	2531968..2532981	-	1014	WP_022645666.1	ABC transporter ATP-binding protein	-
FH485_RS12410	2532999..2534144	-	1146	WP_000047466.1	ABC transporter substrate-binding protein	-
FH485_RS12415	2534389..2535795	-	1407	WP_140028018.1	PLP-dependent aminotransferase family protein	-
FH485_RS12420	2535874..2536290	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
FH485_RS12425	2536336..2536512	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
FH485_RS12430	2536734..2536964	+	231	WP_000491567.1	DUF2554 family protein	-
FH485_RS12435	2537056..2539017	-	1962	WP_023909195.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
FH485_RS12440	2539090..2539626	-	537	WP_000429148.1	DNA-binding transcriptional regulator SutR	-
FH485_RS12445	2539679..2540890	+	1212	WP_071788120.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T127242 WP_000813794.1 NZ_CP040919:c2536512-2536336 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T127242 NZ_CP040919:c2536512-2536336 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTGAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT127242 WP_001270286.1 NZ_CP040919:c2536290-2535874 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT127242 NZ_CP040919:c2536290-2535874 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAACAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB