Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 686007..686842 Replicon chromosome
Accession NZ_CP040919
Organism Escherichia coli strain FC853_EC

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0A1A9A5
Locus tag FH485_RS03325 Protein ID WP_001564063.1
Coordinates 686007..686384 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0A1AEL8
Locus tag FH485_RS03330 Protein ID WP_038432125.1
Coordinates 686474..686842 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH485_RS03295 681135..682283 - 1149 WP_000905920.1 capsule polysaccharide export inner-membrane protein KpsE -
FH485_RS03300 682355..683338 - 984 WP_140027993.1 KpsF/GutQ family sugar-phosphate isomerase -
FH485_RS03305 684149..684319 - 171 Protein_639 IS110 family transposase -
FH485_RS03310 684661..685503 - 843 Protein_640 DUF4942 domain-containing protein -
FH485_RS27810 685588..686010 - 423 WP_001564064.1 DUF957 domain-containing protein -
FH485_RS03325 686007..686384 - 378 WP_001564063.1 TA system toxin CbtA family protein Toxin
FH485_RS03330 686474..686842 - 369 WP_038432125.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
FH485_RS03335 687005..687226 - 222 WP_000692345.1 DUF987 domain-containing protein -
FH485_RS03340 687291..687767 - 477 WP_021553055.1 RadC family protein -
FH485_RS03345 687783..688262 - 480 WP_001564060.1 antirestriction protein -
FH485_RS03350 688528..689346 - 819 WP_001175165.1 DUF945 domain-containing protein -
FH485_RS03355 689436..689669 - 234 WP_001278293.1 DUF905 family protein -
FH485_RS03360 689675..690352 - 678 WP_001564058.1 hypothetical protein -
FH485_RS03365 690503..691183 - 681 WP_001278649.1 WYL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - papX 684534..749758 65224
- flank IS/Tn - - 684149..684304 155


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14094.09 Da        Isoelectric Point: 7.8045

>T127231 WP_001564063.1 NZ_CP040919:c686384-686007 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRSNYRTVNNITRGKHPEAKQ

Download         Length: 378 bp

>T127231 NZ_CP040919:c686384-686007 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGTACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13569.31 Da        Isoelectric Point: 7.0369

>AT127231 WP_038432125.1 NZ_CP040919:c686842-686474 [Escherichia coli]
VSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGNRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSGGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT127231 NZ_CP040919:c686842-686474 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTAACAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A1A9A5

Antitoxin

Source ID Structure
AlphaFold DB A0A0A1AEL8

References