Detailed information of TA system TA127227

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 5797002..5797527
• Replicon: chromosome
• Accession: NZ_CP040916
• Organism: Streptomyces spectabilis strain NRRL 2792

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: FH965_RS25300
• Protein ID: WP_144320728.1
• Coordinates: 5797002..5797265 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S5UWF1
• Locus tag: FH965_RS25305
• Protein ID: WP_019434848.1
• Coordinates: 5797258..5797527 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH965_RS25280	5792913..5793899	+	987	WP_144320725.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
FH965_RS25285	5793987..5794730	+	744	WP_144320726.1	ROK family protein	-
FH965_RS25290	5794819..5795481	-	663	WP_192918194.1	hypothetical protein	-
FH965_RS25295	5795779..5796867	+	1089	WP_144320727.1	redox-regulated ATPase YchF	-
FH965_RS25300	5797002..5797265	-	264	WP_144320728.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FH965_RS25305	5797258..5797527	-	270	WP_019434848.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FH965_RS25310	5797810..5799228	+	1419	WP_144320729.1	amidase	-
FH965_RS25315	5799282..5800520	-	1239	WP_144320730.1	MFS transporter	-
FH965_RS25320	5800580..5801188	-	609	WP_144320731.1	malonic semialdehyde reductase	-
FH965_RS25325	5801370..5801876	+	507	WP_144320732.1	MarR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-58)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10070.51 Da
• Isoelectric Point: 8.5042

>T127227 WP_144320728.1 NZ_CP040916:c5797265-5797002 [Streptomyces spectabilis]
VSEYRTVFRPEAQAELRKIPRDMALRILAKLTELESDPLGFNTTALVSQPDRRRLRVGDYRVVYTIDNGELVVWVVHVGH
RSTVYET

Nucleotide

• Length: 264 bp

>T127227 NZ_CP040916:c5797265-5797002 [Streptomyces spectabilis]
GTGAGTGAGTACCGGACCGTCTTCCGACCGGAGGCGCAGGCCGAGCTTCGGAAGATCCCTCGCGACATGGCGCTGCGCAT
CCTGGCCAAGCTGACCGAGCTGGAGAGCGACCCGCTCGGCTTCAACACCACCGCACTCGTGTCCCAGCCCGACCGACGCC
GTCTGCGCGTCGGCGACTACCGCGTCGTGTACACGATCGACAACGGAGAGCTCGTGGTGTGGGTCGTCCATGTGGGACAC
AGGTCTACCGTTTACGAGACCTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10259.71 Da
• Isoelectric Point: 4.6265

>AT127227 WP_019434848.1 NZ_CP040916:c5797527-5797258 [Streptomyces spectabilis]
MTQPLPIESIRDVRAHLAEVVERADRDDVPTVITRRGKEVAAVVSIEVLRKYQEWEEREINRIIDERMANPAPGIPIEDI
MRETLARGE

Nucleotide

• Length: 270 bp

>AT127227 NZ_CP040916:c5797527-5797258 [Streptomyces spectabilis]
ATGACGCAGCCGCTGCCCATAGAGTCCATCCGCGACGTGCGCGCCCACCTGGCGGAAGTCGTGGAGCGTGCGGACCGCGA
CGACGTACCCACGGTCATCACGCGTCGGGGCAAGGAAGTCGCCGCCGTCGTCTCCATCGAGGTGCTGCGCAAGTATCAGG
AGTGGGAAGAGCGCGAGATCAACCGGATCATCGACGAGCGCATGGCCAACCCGGCACCCGGCATCCCGATCGAGGACATC
ATGAGGGAGACGCTGGCGCGCGGTGAGTGA

Structures

Antitoxin

• AlphaFold DB: S5UWF1