Detailed information of TA system TA127218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1905266..1905951
• Replicon: chromosome
• Accession: NZ_CP040914
• Organism: Mesorhizobium sp. 8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FGU64_RS09230
• Protein ID: WP_136356385.1
• Coordinates: 1905266..1905676 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FGU64_RS09235
• Protein ID: WP_136356387.1
• Coordinates: 1905673..1905951 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGU64_RS09215	1901225..1902451	+	1227	WP_139952724.1	lytic murein transglycosylase	-
FGU64_RS09220	1902504..1903733	+	1230	WP_139952727.1	DUF459 domain-containing protein	-
FGU64_RS09225	1903788..1905242	-	1455	WP_139952730.1	glutamate synthase subunit beta	-
FGU64_RS09230	1905266..1905676	-	411	WP_136356385.1	PIN domain-containing protein	Toxin
FGU64_RS09235	1905673..1905951	-	279	WP_136356387.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FGU64_RS09240	1906001..1910731	-	4731	WP_139952733.1	glutamate synthase large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (18-58)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14888.16 Da
• Isoelectric Point: 7.3185

>T127218 WP_136356385.1 NZ_CP040914:c1905676-1905266 [Mesorhizobium sp. 8]
VTLYLLDTNAVSNLADEPAGPIARHIGKVGPENVATSIIVNGEIEFGLELKQSKRLRAQMEQVFSVLPILPFEYPADRHY
GALRAELKRRGRPIGPNDLLIAAHALALDATLVTANVGEFSRVPGLKIENWLRSQP

Nucleotide

• Length: 411 bp

>T127218 NZ_CP040914:c1905676-1905266 [Mesorhizobium sp. 8]
GTGACGCTTTATCTGTTGGACACGAACGCAGTGTCGAACCTGGCGGATGAGCCGGCCGGACCGATTGCTCGGCATATCGG
CAAGGTCGGGCCAGAAAACGTTGCCACCAGCATCATTGTCAACGGGGAGATCGAATTCGGGCTGGAGTTGAAGCAAAGCA
AGCGACTCAGGGCTCAGATGGAGCAAGTGTTTTCGGTCTTGCCGATCTTGCCCTTTGAGTACCCGGCGGATCGCCACTAC
GGGGCTTTACGGGCAGAGCTCAAGCGACGAGGCCGTCCGATCGGACCGAACGACCTGCTGATCGCAGCACATGCATTGGC
TTTGGACGCCACTCTCGTTACGGCGAATGTCGGGGAGTTTTCCCGCGTCCCGGGCCTCAAGATCGAGAACTGGCTGCGGT
CGCAGCCTTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 4.4109

>AT127218 WP_136356387.1 NZ_CP040914:c1905951-1905673 [Mesorhizobium sp. 8]
MEARMNAHDKDVRTASLFRNGRSQAVRIPKEFEFEGDEVLISRDDTGKLTIEPVRKKPTLLEVLATLEPLAPEDRFPEID
DSDLLPLDDVKL

Nucleotide

• Length: 279 bp

>AT127218 NZ_CP040914:c1905951-1905673 [Mesorhizobium sp. 8]
ATGGAGGCGCGCATGAATGCTCATGACAAGGACGTTCGCACGGCGAGCCTGTTTCGCAACGGGCGTAGCCAGGCCGTGAG
AATACCGAAGGAGTTCGAATTTGAAGGAGACGAGGTTCTTATCAGTCGCGACGATACGGGCAAGCTGACGATTGAGCCGG
TCAGGAAGAAGCCGACGCTGCTGGAAGTGCTGGCGACGCTGGAGCCTCTGGCGCCGGAAGACCGATTCCCGGAAATCGAT
GACAGCGACCTGCTGCCGTTGGACGACGTGAAGCTGTGA