Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 54404..54975 Replicon chromosome
Accession NZ_CP040907
Organism Enterococcus faecium strain FB-1

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag FH486_RS01050 Protein ID WP_002286801.1
Coordinates 54634..54975 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag FH486_RS01045 Protein ID WP_002323011.1
Coordinates 54404..54634 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH486_RS01010 49651..50979 + 1329 WP_008265691.1 FAD-containing oxidoreductase -
FH486_RS01015 51001..51627 + 627 WP_002286816.1 cysteine hydrolase -
FH486_RS01020 51810..52391 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
FH486_RS01030 52999..53574 + 576 WP_002293673.1 SOS response-associated peptidase family protein -
FH486_RS01035 53779..54117 - 339 WP_002286804.1 hypothetical protein -
FH486_RS01045 54404..54634 + 231 WP_002323011.1 hypothetical protein Antitoxin
FH486_RS01050 54634..54975 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FH486_RS01055 55825..56010 + 186 WP_002304207.1 hypothetical protein -
FH486_RS01060 56367..57529 + 1163 WP_086956687.1 IS3 family transposase -
FH486_RS01065 57884..58078 + 195 WP_002295146.1 hypothetical protein -
FH486_RS01070 58236..58518 + 283 Protein_55 transposase -
FH486_RS01080 58762..59592 - 831 WP_002286789.1 manganese catalase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T127210 WP_002286801.1 NZ_CP040907:54634-54975 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T127210 NZ_CP040907:54634-54975 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT127210 WP_002323011.1 NZ_CP040907:54404-54634 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT127210 NZ_CP040907:54404-54634 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References