Detailed information of TA system TA127209

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 1636..2286
• Replicon: plasmid punnamed
• Accession: NZ_CP040906
• Organism: Enterococcus faecium strain FB-1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: FH486_RS00020
• Protein ID: WP_065305698.1
• Coordinates: 2101..2286 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A829EWE8
• Locus tag: FH486_RS00015
• Protein ID: WP_002305052.1
• Coordinates: 1636..2067 (-)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH486_RS00010	390..1451	+	1062	WP_065305699.1	hypothetical protein	-
FH486_RS00015	1636..2067	-	432	WP_002305052.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
FH486_RS00020	2101..2286	-	186	WP_065305698.1	type II toxin-antitoxin system HicA family toxin	Toxin
FH486_RS00025	2410..2589	-	180	WP_065305697.1	hypothetical protein	-
FH486_RS00035	2970..3677	-	708	WP_065305695.1	hypothetical protein	-
FH486_RS00040	3670..4107	-	438	WP_065305694.1	hypothetical protein	-
FH486_RS00045	4122..4373	-	252	WP_065305693.1	hypothetical protein	-
FH486_RS00050	4373..4579	-	207	WP_065305692.1	DNA helicase UvrA	-
FH486_RS00055	5023..5283	-	261	WP_049055261.1	hypothetical protein	-
FH486_RS00060	5358..5561	+	204	WP_065305691.1	putative holin-like toxin	-
FH486_RS00065	5721..5978	-	258	WP_065305690.1	hypothetical protein	-
FH486_RS00070	6000..6473	-	474	WP_065305689.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..133232	133232

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (10-104)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6997.21 Da
• Isoelectric Point: 10.8134

>T127209 WP_065305698.1 NZ_CP040906:c2286-2101 [Enterococcus faecium]
MPLTGKEMLKLLKKNGWVERRQEGSHHHLYKDGVRITVPVHANQDLGRGLERKILKDAGLK

Nucleotide

• Length: 186 bp

>T127209 NZ_CP040906:c2286-2101 [Enterococcus faecium]
ATGCCACTTACGGGAAAAGAAATGCTTAAGTTATTGAAAAAAAATGGCTGGGTTGAGCGAAGGCAAGAGGGATCTCATCA
TCATCTTTATAAGGATGGTGTTAGAATCACTGTTCCTGTTCATGCCAATCAAGATCTTGGCCGAGGACTTGAGCGAAAAA
TTTTGAAGGATGCGGGATTGAAATAA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 15811.92 Da
• Isoelectric Point: 4.1638

>AT127209 WP_002305052.1 NZ_CP040906:c2067-1636 [Enterococcus faecium]
MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYGIAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDD
SFTTLIRVDVAKYLKDTELVKKTLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN

Nucleotide

• Length: 432 bp

>AT127209 NZ_CP040906:c2067-1636 [Enterococcus faecium]
ATGTTAAGTGAGCCTTTAGCAAAAACCTATCCTGCTATTTTTAGCCCAGAAGAAGGTGGTGGGTACTTTATTGAATTTCC
TGATGTTCAAGGTGCTTACACAGGAATTAACGAAGACGATATATCTTATGGGATTGCTATGGCACAAGAGGTGTTAGGTA
TGGTTTTAGCAGATTATATTGAGCATGAAGATCTACTTCCAGAACCAACACCAATCAATAAGATTTCTGTTGAAGATGAT
TCTTTTACTACTTTAATTCGTGTAGATGTTGCCAAATATTTGAAAGACACAGAACTTGTCAAAAAAACCTTAACAATTCC
TCAATGGGCGGATAAGTTAGGCAAAAGGGCTGGCATAAATTTTTCAGTCCTTCTAACAGAATCAATTGCAGATAAAGCAG
ATAACATTCTTCGTTCTGGAAGAAATAATTAG

Structures

Antitoxin

• AlphaFold DB: A0A829EWE8