Detailed information of TA system TA127199

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 196166..196737
• Replicon: chromosome
• Accession: NZ_CP040898
• Organism: Enterococcus faecalis strain HA-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FHO10_RS01355
• Protein ID: WP_002354774.1
• Coordinates: 196396..196737 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: FHO10_RS01350
• Protein ID: WP_002354773.1
• Coordinates: 196166..196396 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FHO10_RS01325	191540..193156	-	1617	WP_002378864.1	phosphatase PAP2/LCP family protein	-
FHO10_RS01335	193841..194479	+	639	WP_002378863.1	lytic polysaccharide monooxygenase	-
FHO10_RS01340	194646..195638	-	993	WP_002378862.1	biotin--[acetyl-CoA-carboxylase] ligase	-
FHO10_RS01345	195777..195992	+	216	WP_002354768.1	zinc ribbon domain-containing protein	-
FHO10_RS01350	196166..196396	+	231	WP_002354773.1	hypothetical protein	Antitoxin
FHO10_RS01355	196396..196737	+	342	WP_002354774.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FHO10_RS01365	197108..200722	+	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13156.40 Da
• Isoelectric Point: 9.3984

>T127199 WP_002354774.1 NZ_CP040898:196396-196737 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T127199 NZ_CP040898:196396-196737 [Enterococcus faecalis]
ATGAATGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT127199 WP_002354773.1 NZ_CP040898:196166-196396 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT127199 NZ_CP040898:196166-196396 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812