Detailed information of TA system TA127177

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3635327..3635952
• Replicon: chromosome
• Accession: NZ_CP040886
• Organism: Escherichia coli strain K71-77

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FHY70_RS19560
• Protein ID: WP_000911330.1
• Coordinates: 3635554..3635952 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: FHY70_RS19555
• Protein ID: WP_000450524.1
• Coordinates: 3635327..3635554 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FHY70_RS19530	3631130..3631600	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
FHY70_RS19535	3631600..3632172	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
FHY70_RS19540	3632318..3633196	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FHY70_RS19545	3633213..3634247	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
FHY70_RS19550	3634460..3635173	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
FHY70_RS19555	3635327..3635554	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
FHY70_RS19560	3635554..3635952	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
FHY70_RS19565	3636099..3636962	+	864	WP_060503976.1	neutral zinc metallopeptidase	-
FHY70_RS19570	3636977..3638992	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
FHY70_RS19575	3639066..3639764	+	699	WP_000679823.1	esterase	-
FHY70_RS19580	3639874..3640074	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T127177 WP_000911330.1 NZ_CP040886:3635554-3635952 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T127177 NZ_CP040886:3635554-3635952 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT127177 WP_000450524.1 NZ_CP040886:3635327-3635554 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT127177 NZ_CP040886:3635327-3635554 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33