Detailed information of TA system TA127167

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1755435..1756267
• Replicon: chromosome
• Accession: NZ_CP040886
• Organism: Escherichia coli strain K71-77

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2R9V8K2
• Locus tag: FHY70_RS10445
• Protein ID: WP_001468432.1
• Coordinates: 1755893..1756267 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: Q1R2L5
• Locus tag: FHY70_RS10440
• Protein ID: WP_001285575.1
• Coordinates: 1755435..1755803 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FHY70_RS10405	1751328..1752008	+	681	WP_001282919.1	WYL domain-containing protein	-
FHY70_RS10410	1752156..1752833	+	678	WP_001097301.1	hypothetical protein	-
FHY70_RS10415	1752839..1753072	+	234	WP_001278283.1	DUF905 family protein	-
FHY70_RS10420	1753162..1753980	+	819	WP_001175160.1	DUF945 domain-containing protein	-
FHY70_RS10425	1754071..1754556	+	486	WP_000213697.1	antirestriction protein	-
FHY70_RS10430	1754571..1755047	+	477	WP_001298074.1	RadC family protein	-
FHY70_RS10435	1755134..1755355	+	222	WP_000692350.1	DUF987 domain-containing protein	-
FHY70_RS10440	1755435..1755803	+	369	WP_001285575.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FHY70_RS10445	1755893..1756267	+	375	WP_001468432.1	TA system toxin CbtA family protein	Toxin
FHY70_RS10450	1756264..1756752	+	489	WP_000777545.1	hypothetical protein	-
FHY70_RS10455	1756764..1756961	+	198	WP_000839281.1	DUF957 domain-containing protein	-
FHY70_RS10460	1757058..1757627	+	570	WP_001290252.1	DUF4942 domain-containing protein	-
FHY70_RS10465	1758376..1759914	+	1539	WP_001187182.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1737008..1767727	30719

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14102.04 Da
• Isoelectric Point: 7.3526

>T127167 WP_001468432.1 NZ_CP040886:1755893-1756267 [Escherichia coli]
MNTLPDTHIREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T127167 NZ_CP040886:1755893-1756267 [Escherichia coli]
ATGAACACATTACCCGATACACACATACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13709.52 Da
• Isoelectric Point: 6.6258

>AT127167 WP_001285575.1 NZ_CP040886:1755435-1755803 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT127167 NZ_CP040886:1755435-1755803 [Escherichia coli]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A2R9V8K2

Antitoxin

• AlphaFold DB: A0A454ABF5