Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 31708..32233 Replicon plasmid pK71-77-2
Accession NZ_CP040885
Organism Escherichia coli strain K71-77

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag FHY70_RS01120 Protein ID WP_001159871.1
Coordinates 31928..32233 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag FHY70_RS01115 Protein ID WP_000813630.1
Coordinates 31708..31926 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FHY70_RS01085 26918..27604 + 687 Protein_26 IS1 family transposase -
FHY70_RS01090 27858..28880 - 1023 WP_000361402.1 helicase UvrD -
FHY70_RS01095 28865..30430 - 1566 WP_001128474.1 AAA family ATPase -
FHY70_RS01100 30505..30921 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
FHY70_RS01105 30918..31148 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
FHY70_RS01115 31708..31926 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FHY70_RS01120 31928..32233 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
FHY70_RS01125 32234..33040 + 807 WP_000016970.1 site-specific integrase -
FHY70_RS01130 33762..34517 + 756 WP_000852146.1 replication initiation protein RepE -
FHY70_RS01135 35105..36271 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / aac(6')-Ib-cr / blaOXA-1 iucA / iucB / iucC / iucD / iutA 1..117590 117590
- flank IS/Tn - - 27257..27604 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T127157 WP_001159871.1 NZ_CP040885:31928-32233 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T127157 NZ_CP040885:31928-32233 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT127157 WP_000813630.1 NZ_CP040885:31708-31926 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT127157 NZ_CP040885:31708-31926 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References