Detailed information of TA system TA127149

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1241176..1242093
• Replicon: chromosome
• Accession: NZ_CP040881
• Organism: Bacillus sp. HNA3

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: FHJ82_RS06465
• Protein ID: WP_007407256.1
• Coordinates: 1241347..1242093 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: FHJ82_RS06460
• Protein ID: WP_003154807.1
• Coordinates: 1241176..1241346 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FHJ82_RS06425	1236409..1238031	+	1623	WP_032874601.1	pyocin knob domain-containing protein	-
FHJ82_RS06430	1238044..1238415	+	372	WP_032874603.1	YomQ/XkdW protein, phage-like element PBSX	-
FHJ82_RS06435	1238420..1238617	+	198	WP_032874605.1	XkdX family protein	-
FHJ82_RS06440	1238674..1239435	+	762	WP_032874607.1	hypothetical protein	-
FHJ82_RS06445	1239487..1239750	+	264	WP_003154815.1	hemolysin XhlA family protein	-
FHJ82_RS06450	1239764..1240027	+	264	WP_003154813.1	phage holin	-
FHJ82_RS06455	1240041..1240919	+	879	WP_032874609.1	N-acetylmuramoyl-L-alanine amidase	-
FHJ82_RS06460	1241176..1241346	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
FHJ82_RS06465	1241347..1242093	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
FHJ82_RS06470	1242198..1243196	-	999	WP_032874611.1	inorganic phosphate transporter	-
FHJ82_RS06475	1243209..1243826	-	618	WP_032874614.1	DUF47 domain-containing protein	-
FHJ82_RS06480	1244112..1245428	-	1317	WP_032874616.1	amino acid permease	-
FHJ82_RS06485	1245750..1246700	+	951	WP_032874618.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1180435..1299943	119508

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T127149 WP_007407256.1 NZ_CP040881:c1242093-1241347 [Bacillus sp. HNA3]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T127149 NZ_CP040881:c1242093-1241347 [Bacillus sp. HNA3]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTGAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCTGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT127149 WP_003154807.1 NZ_CP040881:c1241346-1241176 [Bacillus sp. HNA3]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT127149 NZ_CP040881:c1241346-1241176 [Bacillus sp. HNA3]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14