Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 489960..490597 Replicon chromosome
Accession NZ_CP040881
Organism Bacillus sp. HNA3

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag FHJ82_RS02500 Protein ID WP_003156187.1
Coordinates 490247..490597 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag FHJ82_RS02495 Protein ID WP_003156188.1
Coordinates 489960..490241 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FHJ82_RS02475 486325..486924 - 600 WP_032872997.1 rhomboid family intramembrane serine protease -
FHJ82_RS02480 487017..487382 + 366 WP_007609580.1 holo-ACP synthase -
FHJ82_RS02485 487547..488554 + 1008 WP_032872999.1 outer membrane lipoprotein carrier protein LolA -
FHJ82_RS02490 488671..489840 + 1170 WP_032873001.1 alanine racemase -
FHJ82_RS02495 489960..490241 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
FHJ82_RS02500 490247..490597 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
FHJ82_RS02505 490715..491536 + 822 WP_003156182.1 STAS domain-containing protein -
FHJ82_RS02510 491541..491906 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
FHJ82_RS02515 491909..492310 + 402 WP_003156178.1 anti-sigma regulatory factor -
FHJ82_RS02520 492322..493329 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
FHJ82_RS02525 493393..493722 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
FHJ82_RS02530 493719..494201 + 483 WP_007609591.1 anti-sigma B factor RsbW -
FHJ82_RS02535 494167..494955 + 789 WP_032873003.1 RNA polymerase sigma factor SigB -
FHJ82_RS02540 494955..495557 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T127148 WP_003156187.1 NZ_CP040881:490247-490597 [Bacillus sp. HNA3]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T127148 NZ_CP040881:490247-490597 [Bacillus sp. HNA3]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT127148 WP_003156188.1 NZ_CP040881:489960-490241 [Bacillus sp. HNA3]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT127148 NZ_CP040881:489960-490241 [Bacillus sp. HNA3]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References