Detailed information of TA system TA127145

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 52340..52990
• Replicon: plasmid punnamed2
• Accession: NZ_CP040876
• Organism: Enterococcus faecium strain DB-1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: FHJ99_RS14325
• Protein ID: WP_065305698.1
• Coordinates: 52340..52525 (+)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A829EWE8
• Locus tag: FHJ99_RS14330
• Protein ID: WP_002305052.1
• Coordinates: 52559..52990 (+)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FHJ99_RS14275	48153..48626	+	474	WP_065305689.1	single-stranded DNA-binding protein	-
FHJ99_RS14280	48648..48905	+	258	WP_065305690.1	hypothetical protein	-
FHJ99_RS14285	49065..49268	-	204	WP_065305691.1	putative holin-like toxin	-
FHJ99_RS14290	49343..49603	+	261	WP_049055261.1	hypothetical protein	-
FHJ99_RS14295	50047..50253	+	207	WP_065305692.1	DNA helicase UvrA	-
FHJ99_RS14300	50253..50504	+	252	WP_065305693.1	hypothetical protein	-
FHJ99_RS14305	50519..50956	+	438	WP_065305694.1	hypothetical protein	-
FHJ99_RS14310	50949..51656	+	708	WP_065305695.1	hypothetical protein	-
FHJ99_RS14320	52037..52216	+	180	WP_065305697.1	hypothetical protein	-
FHJ99_RS14325	52340..52525	+	186	WP_065305698.1	type II toxin-antitoxin system HicA family toxin	Toxin
FHJ99_RS14330	52559..52990	+	432	WP_002305052.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
FHJ99_RS14335	53175..54236	-	1062	WP_065305699.1	hypothetical protein	-
FHJ99_RS14340	54254..54853	-	600	WP_065305700.1	recombinase family protein	-
FHJ99_RS14345	55082..56134	+	1053	WP_065305701.1	TIGR00341 family protein	-
FHJ99_RS14350	56705..57295	+	591	WP_065305702.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..110319	110319

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (10-104)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6997.21 Da
• Isoelectric Point: 10.8134

>T127145 WP_065305698.1 NZ_CP040876:52340-52525 [Enterococcus faecium]
MPLTGKEMLKLLKKNGWVERRQEGSHHHLYKDGVRITVPVHANQDLGRGLERKILKDAGLK

Nucleotide

• Length: 186 bp

>T127145 NZ_CP040876:52340-52525 [Enterococcus faecium]
ATGCCACTTACGGGAAAAGAAATGCTTAAGTTATTGAAAAAAAATGGCTGGGTTGAGCGAAGGCAAGAGGGATCTCATCA
TCATCTTTATAAGGATGGTGTTAGAATCACTGTTCCTGTTCATGCCAATCAAGATCTTGGCCGAGGACTTGAGCGAAAAA
TTTTGAAGGATGCGGGATTGAAATAA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 15811.92 Da
• Isoelectric Point: 4.1638

>AT127145 WP_002305052.1 NZ_CP040876:52559-52990 [Enterococcus faecium]
MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYGIAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDD
SFTTLIRVDVAKYLKDTELVKKTLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN

Nucleotide

• Length: 432 bp

>AT127145 NZ_CP040876:52559-52990 [Enterococcus faecium]
ATGTTAAGTGAGCCTTTAGCAAAAACCTATCCTGCTATTTTTAGCCCAGAAGAAGGTGGTGGGTACTTTATTGAATTTCC
TGATGTTCAAGGTGCTTACACAGGAATTAACGAAGACGATATATCTTATGGGATTGCTATGGCACAAGAGGTGTTAGGTA
TGGTTTTAGCAGATTATATTGAGCATGAAGATCTACTTCCAGAACCAACACCAATCAATAAGATTTCTGTTGAAGATGAT
TCTTTTACTACTTTAATTCGTGTAGATGTTGCCAAATATTTGAAAGACACAGAACTTGTCAAAAAAACCTTAACAATTCC
TCAATGGGCGGATAAGTTAGGCAAAAGGGCTGGCATAAATTTTTCAGTCCTTCTAACAGAATCAATTGCAGATAAAGCAG
ATAACATTCTTCGTTCTGGAAGAAATAATTAG

Structures

Antitoxin

• AlphaFold DB: A0A829EWE8