Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 984466..985037 Replicon chromosome
Accession NZ_CP040875
Organism Enterococcus faecium strain DB-1

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag FHJ99_RS05380 Protein ID WP_002286801.1
Coordinates 984696..985037 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag FHJ99_RS05375 Protein ID WP_002323011.1
Coordinates 984466..984696 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FHJ99_RS05340 979713..981041 + 1329 WP_008265691.1 FAD-containing oxidoreductase -
FHJ99_RS05345 981063..981689 + 627 WP_002286816.1 cysteine hydrolase -
FHJ99_RS05350 981872..982453 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
FHJ99_RS05360 983061..983636 + 576 WP_002293673.1 SOS response-associated peptidase family protein -
FHJ99_RS05365 983841..984179 - 339 WP_002286804.1 hypothetical protein -
FHJ99_RS05375 984466..984696 + 231 WP_002323011.1 hypothetical protein Antitoxin
FHJ99_RS05380 984696..985037 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FHJ99_RS05385 985887..986072 + 186 WP_002304207.1 hypothetical protein -
FHJ99_RS05390 986429..987591 + 1163 WP_086956687.1 IS3 family transposase -
FHJ99_RS05395 987946..988140 + 195 WP_002295146.1 hypothetical protein -
FHJ99_RS05400 988298..988580 + 283 Protein_881 transposase -
FHJ99_RS05410 988824..989654 - 831 WP_002286789.1 manganese catalase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T127144 WP_002286801.1 NZ_CP040875:984696-985037 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T127144 NZ_CP040875:984696-985037 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT127144 WP_002323011.1 NZ_CP040875:984466-984696 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT127144 NZ_CP040875:984466-984696 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References