Detailed information of TA system TA127131

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-dnstrm_HI1420
• Location: 908288..908877
• Replicon: chromosome
• Accession: NZ_CP040863
• Organism: Rodentibacter heylii strain G1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: FEE42_RS04500
• Protein ID: WP_164027814.1
• Coordinates: 908288..908587 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: FEE42_RS04505
• Protein ID: WP_077493439.1
• Coordinates: 908584..908877 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEE42_RS04460	903714..904217	-	504	WP_164027808.1	terminase small subunit	-
FEE42_RS04465	904235..905635	-	1401	WP_164027809.1	site-specific DNA-methyltransferase	-
FEE42_RS04470	905838..906176	-	339	WP_164027810.1	DUF2570 family protein	-
FEE42_RS04475	906149..906697	-	549	WP_164027811.1	lysozyme	-
FEE42_RS04480	906690..906935	-	246	WP_164027812.1	hypothetical protein	-
FEE42_RS04485	907041..907601	+	561	WP_164027813.1	hypothetical protein	-
FEE42_RS04490	907655..907924	-	270	WP_152634407.1	hypothetical protein	-
FEE42_RS04500	908288..908587	+	300	WP_164027814.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FEE42_RS04505	908584..908877	+	294	WP_077493439.1	putative addiction module antidote protein	Antitoxin
FEE42_RS04510	908917..909285	-	369	WP_164027815.1	antitermination protein	-
FEE42_RS04515	909275..909835	-	561	WP_164027816.1	recombination protein NinG	-
FEE42_RS04520	909922..910104	+	183	WP_135968557.1	type II toxin-antitoxin system HicA family toxin	-
FEE42_RS04525	910133..910588	+	456	WP_135968556.1	type II toxin-antitoxin system HicB family antitoxin	-
FEE42_RS04530	910870..911529	+	660	WP_164027817.1	KilA-N domain-containing protein	-
FEE42_RS04535	911566..911973	-	408	WP_164028809.1	recombination protein NinB	-
FEE42_RS04540	911984..912526	-	543	WP_164027818.1	adenine methyltransferase	-
FEE42_RS04545	912523..913158	-	636	WP_167514392.1	replication P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	875164..977547	102383

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (45-89)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11274.10 Da
• Isoelectric Point: 10.2800

>T127131 WP_164027814.1 NZ_CP040863:908288-908587 [Rodentibacter heylii]
MTIQIKTTLTFDNWLSKLKNIRAKAKINARIKRLQFGNFGDIKSVNDGIFELRIDEGQGYRIYLKNQNGVLVFLLCGGDK
STQEQDIKKAKQLAEELGL

Nucleotide

• Length: 300 bp

>T127131 NZ_CP040863:908288-908587 [Rodentibacter heylii]
ATGACAATCCAAATTAAAACAACTTTAACTTTTGATAACTGGCTGAGCAAACTAAAGAATATTCGTGCTAAAGCTAAAAT
CAATGCACGAATCAAACGTTTACAGTTCGGAAACTTTGGCGATATTAAAAGTGTAAATGATGGCATTTTTGAATTACGCA
TAGATGAAGGACAAGGCTATCGAATTTATCTGAAAAATCAAAACGGTGTTTTAGTATTTTTACTATGTGGCGGAGACAAA
TCCACACAAGAACAAGATATCAAGAAAGCAAAACAACTTGCAGAGGAATTAGGATTATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10806.29 Da
• Isoelectric Point: 4.4488

>AT127131 WP_077493439.1 NZ_CP040863:908584-908877 [Rodentibacter heylii]
MTEQLKDFDIAEHLTSEEEIQLYLNEILQEDNVELILSALGDIARARNMSQIARDAGISREGLYKALSGSGNPTFATVMK
VMKALNLKFQVQQSSFA

Nucleotide

• Length: 294 bp

>AT127131 NZ_CP040863:908584-908877 [Rodentibacter heylii]
ATGACAGAACAATTAAAAGATTTTGATATTGCAGAACATCTCACTTCCGAAGAGGAAATTCAGCTTTATCTCAACGAAAT
TTTACAAGAAGACAATGTTGAACTTATTTTATCTGCGCTTGGAGATATTGCCCGAGCACGTAATATGAGCCAAATTGCCC
GTGATGCAGGAATCAGCCGTGAAGGGCTTTATAAAGCATTATCCGGTTCAGGTAACCCAACTTTTGCTACCGTGATGAAA
GTTATGAAAGCATTAAACCTCAAATTCCAAGTTCAACAGTCGAGTTTTGCTTAA