Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 206830..207566 Replicon plasmid unnamed3
Accession NZ_CP040862
Organism Klebsiella pneumoniae strain Xen39

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag FEE38_RS28990 Protein ID WP_003026803.1
Coordinates 207084..207566 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag FEE38_RS28985 Protein ID WP_003026799.1
Coordinates 206830..207096 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEE38_RS28945 202193..203731 + 1539 WP_004196907.1 IS66-like element ISEc22 family transposase -
FEE38_RS28950 204021..204284 + 264 WP_009310051.1 hypothetical protein -
FEE38_RS28955 204281..204847 + 567 WP_009310052.1 hypothetical protein -
FEE38_RS28960 204878..205372 + 495 WP_009310053.1 hypothetical protein -
FEE38_RS28965 205416..205784 + 369 WP_009310054.1 hypothetical protein -
FEE38_RS28970 205815..206018 + 204 WP_004098931.1 hemolysin expression modulator Hha -
FEE38_RS28975 206067..206324 + 258 WP_004098928.1 hypothetical protein -
FEE38_RS28980 206400..206654 + 255 WP_004118702.1 hypothetical protein -
FEE38_RS28985 206830..207096 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
FEE38_RS28990 207084..207566 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
FEE38_RS28995 207725..208989 - 1265 Protein_230 IS3 family transposase -
FEE38_RS29000 209077..210039 - 963 WP_004152113.1 M48 family metalloprotease -
FEE38_RS29005 210026..210775 - 750 WP_009483782.1 phosphate-starvation-inducible PsiE family protein -
FEE38_RS29010 211013..211210 - 198 WP_004152115.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ 1..263991 263991
- inside IScluster/Tn - - 201396..208762 7366


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T127130 WP_003026803.1 NZ_CP040862:207084-207566 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T127130 NZ_CP040862:207084-207566 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT127130 WP_003026799.1 NZ_CP040862:206830-207096 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT127130 NZ_CP040862:206830-207096 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References