Detailed information of TA system TA127129

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 172828..173471
• Replicon: plasmid unnamed3
• Accession: NZ_CP040862
• Organism: Klebsiella pneumoniae strain Xen39

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: FEE38_RS28795
• Protein ID: WP_001044770.1
• Coordinates: 173055..173471 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: FEE38_RS28790
• Protein ID: WP_001261282.1
• Coordinates: 172828..173058 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEE38_RS28740	168377..168490	+	114	WP_014343462.1	hypothetical protein	-
FEE38_RS28745	168619..168876	+	258	WP_009310077.1	hypothetical protein	-
FEE38_RS28750	168934..169713	-	780	WP_023287113.1	site-specific integrase	-
FEE38_RS28760	169911..170927	-	1017	WP_009309921.1	hypothetical protein	-
FEE38_RS28765	170961..171296	-	336	WP_009309920.1	hypothetical protein	-
FEE38_RS28770	171346..171657	-	312	WP_009309919.1	hypothetical protein	-
FEE38_RS28775	171715..172020	-	306	WP_009309918.1	type II toxin-antitoxin system toxin CcdB	-
FEE38_RS28780	172022..172240	-	219	WP_006788213.1	type II toxin-antitoxin system antitoxin CcdA	-
FEE38_RS28785	172410..172871	-	462	WP_163589431.1	hypothetical protein	-
FEE38_RS28790	172828..173058	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FEE38_RS28795	173055..173471	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
FEE38_RS28800	173545..175107	+	1563	WP_009309907.1	AAA family ATPase	-
FEE38_RS28805	175092..176114	+	1023	WP_000361404.1	helicase UvrD	-
FEE38_RS28815	176659..177567	+	909	WP_032451458.1	HNH endonuclease	-
FEE38_RS28820	177753..178103	-	351	WP_004187110.1	DUF305 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ	1..263991	263991
	-	flank	IS/Tn	-	-	178184..179152	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T127129 WP_001044770.1 NZ_CP040862:173055-173471 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T127129 NZ_CP040862:173055-173471 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT127129 WP_001261282.1 NZ_CP040862:172828-173058 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT127129 NZ_CP040862:172828-173058 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3