Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 171715..172240 Replicon plasmid unnamed3
Accession NZ_CP040862
Organism Klebsiella pneumoniae strain Xen39

Toxin (Protein)


Gene name ccdB Uniprot ID A0A7H9GHC5
Locus tag FEE38_RS28775 Protein ID WP_009309918.1
Coordinates 171715..172020 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag FEE38_RS28780 Protein ID WP_006788213.1
Coordinates 172022..172240 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEE38_RS28735 166877..167845 + 969 WP_000654811.1 IS5 family transposase -
FEE38_RS28740 168377..168490 + 114 WP_014343462.1 hypothetical protein -
FEE38_RS28745 168619..168876 + 258 WP_009310077.1 hypothetical protein -
FEE38_RS28750 168934..169713 - 780 WP_023287113.1 site-specific integrase -
FEE38_RS28760 169911..170927 - 1017 WP_009309921.1 hypothetical protein -
FEE38_RS28765 170961..171296 - 336 WP_009309920.1 hypothetical protein -
FEE38_RS28770 171346..171657 - 312 WP_009309919.1 hypothetical protein -
FEE38_RS28775 171715..172020 - 306 WP_009309918.1 type II toxin-antitoxin system toxin CcdB Toxin
FEE38_RS28780 172022..172240 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FEE38_RS28785 172410..172871 - 462 WP_163589431.1 hypothetical protein -
FEE38_RS28790 172828..173058 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
FEE38_RS28795 173055..173471 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin -
FEE38_RS28800 173545..175107 + 1563 WP_009309907.1 AAA family ATPase -
FEE38_RS28805 175092..176114 + 1023 WP_000361404.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ 1..263991 263991


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11571.24 Da        Isoelectric Point: 6.4661

>T127128 WP_009309918.1 NZ_CP040862:c172020-171715 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVVPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T127128 NZ_CP040862:c172020-171715 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGGTCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT127128 WP_006788213.1 NZ_CP040862:c172240-172022 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT127128 NZ_CP040862:c172240-172022 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGGCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCCGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H9GHC5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References