Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2831922..2832547 Replicon chromosome
Accession NZ_CP040861
Organism Klebsiella pneumoniae strain Xen39

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag FEE38_RS14495 Protein ID WP_002882817.1
Coordinates 2831922..2832305 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag FEE38_RS14500 Protein ID WP_004150355.1
Coordinates 2832305..2832547 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEE38_RS14480 2829288..2830190 + 903 WP_117096740.1 formate dehydrogenase subunit beta -
FEE38_RS14485 2830187..2830822 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
FEE38_RS14490 2830819..2831748 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
FEE38_RS14495 2831922..2832305 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
FEE38_RS14500 2832305..2832547 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
FEE38_RS14505 2832752..2833669 + 918 WP_021466676.1 alpha/beta hydrolase -
FEE38_RS14510 2833683..2834624 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
FEE38_RS14515 2834669..2835106 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
FEE38_RS14520 2835103..2835963 - 861 WP_004146232.1 virulence factor BrkB family protein -
FEE38_RS14525 2835957..2836556 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T127115 WP_002882817.1 NZ_CP040861:c2832305-2831922 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T127115 NZ_CP040861:c2832305-2831922 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT127115 WP_004150355.1 NZ_CP040861:c2832547-2832305 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT127115 NZ_CP040861:c2832547-2832305 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References