Detailed information of TA system TA127108

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 86811..87310
• Replicon: plasmid unnamed1
• Accession: NZ_CP040855
• Organism: Lactobacillus johnsonii strain G2A

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: FEE39_RS09805
• Protein ID: WP_163588922.1
• Coordinates: 86811..87092 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: FEE39_RS09810
• Protein ID: WP_163588923.1
• Coordinates: 87095..87310 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEE39_RS09760	82273..82581	+	309	WP_163588914.1	type II toxin-antitoxin system HicB family antitoxin	-
FEE39_RS09765	82766..82948	+	183	WP_163588915.1	type II toxin-antitoxin system HicA family toxin	-
FEE39_RS09770	82981..83382	+	402	WP_163588916.1	type II toxin-antitoxin system HicB family antitoxin	-
FEE39_RS09775	83522..83968	-	447	WP_163588917.1	hypothetical protein	-
FEE39_RS09780	83984..84568	-	585	WP_163588918.1	hypothetical protein	-
FEE39_RS09785	84585..85190	-	606	WP_163588919.1	hypothetical protein	-
FEE39_RS09790	85174..85407	-	234	WP_163588920.1	hypothetical protein	-
FEE39_RS09795	85902..86174	+	273	WP_163588955.1	HU family DNA-binding protein	-
FEE39_RS09800	86308..86520	+	213	WP_163588921.1	hypothetical protein	-
FEE39_RS09805	86811..87092	-	282	WP_163588922.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FEE39_RS09810	87095..87310	-	216	WP_163588923.1	hypothetical protein	Antitoxin
FEE39_RS09815	87627..88799	-	1173	WP_163588924.1	methyltransferase domain-containing protein	-
FEE39_RS10660	88908..89060	-	153	WP_204305257.1	hypothetical protein	-
FEE39_RS09825	89351..90685	-	1335	WP_135014334.1	transposase	-
FEE39_RS09830	90784..91248	+	465	WP_087284950.1	IS200/IS605-like element ISLjo5 family transposase	-
FEE39_RS09835	91214..91531	-	318	WP_163588925.1	hypothetical protein	-
FEE39_RS09840	91568..91942	-	375	WP_163588926.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..127062	127062

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-84)

Antitoxin

• Protein of unknown function DUF6290 (1-69)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11237.98 Da
• Isoelectric Point: 10.6078

>T127108 WP_163588922.1 NZ_CP040855:c87092-86811 [Lactobacillus johnsonii]
MKYTWEFNKKAFKQFKKELDKPVQRRIIEWLDSHIEGSNNPRIWGKALEGNYQTLWRYRIGSYRIIADIKDDVFTVVVVK
AGKRNDVYKQKSR

Nucleotide

• Length: 282 bp

>T127108 NZ_CP040855:c87092-86811 [Lactobacillus johnsonii]
TTGAAATACACATGGGAATTTAACAAGAAGGCATTTAAGCAATTTAAAAAAGAACTTGATAAACCCGTTCAAAGGAGAAT
TATTGAATGGTTAGATTCACATATTGAAGGTAGCAATAATCCTAGAATTTGGGGTAAGGCTCTTGAGGGTAATTATCAAA
CCCTATGGCGATATCGAATAGGAAGCTATAGAATTATTGCTGATATTAAAGATGACGTTTTTACAGTTGTTGTTGTTAAA
GCTGGAAAAAGAAATGATGTTTATAAGCAAAAAAGCCGTTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7981.00 Da
• Isoelectric Point: 5.0336

>AT127108 WP_163588923.1 NZ_CP040855:c87310-87095 [Lactobacillus johnsonii]
MKSTTIRFNEDILNIIHKQAALDGQNSTQFMRNAILEKLEDSLDYQDAIKNLKGSDGETVSRADVKKELGI

Nucleotide

• Length: 216 bp

>AT127108 NZ_CP040855:c87310-87095 [Lactobacillus johnsonii]
ATGAAGTCAACTACTATCAGATTTAATGAAGATATTTTGAATATTATTCATAAACAAGCTGCATTAGACGGACAAAACTC
CACTCAGTTTATGAGAAATGCTATTTTAGAAAAGTTAGAGGACAGCTTAGATTATCAAGATGCAATTAAAAACCTTAAAG
GTTCTGATGGAGAAACAGTTTCTCGTGCTGATGTAAAGAAAGAACTAGGTATATAA