Detailed information of TA system TA127107

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 82766..83382
• Replicon: plasmid unnamed1
• Accession: NZ_CP040855
• Organism: Lactobacillus johnsonii strain G2A

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: FEE39_RS09765
• Protein ID: WP_163588915.1
• Coordinates: 82766..82948 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: FEE39_RS09770
• Protein ID: WP_163588916.1
• Coordinates: 82981..83382 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEE39_RS09735	78036..78494	-	459	WP_163588909.1	hypothetical protein	-
FEE39_RS09740	78514..79728	-	1215	WP_163588910.1	ParM/StbA family protein	-
FEE39_RS09745	80236..80463	-	228	WP_163588911.1	hypothetical protein	-
FEE39_RS09750	80482..81357	-	876	WP_163588912.1	ParA family protein	-
FEE39_RS09755	81514..81780	+	267	WP_163588913.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FEE39_RS09760	82273..82581	+	309	WP_163588914.1	type II toxin-antitoxin system HicB family antitoxin	-
FEE39_RS09765	82766..82948	+	183	WP_163588915.1	type II toxin-antitoxin system HicA family toxin	Toxin
FEE39_RS09770	82981..83382	+	402	WP_163588916.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
FEE39_RS09775	83522..83968	-	447	WP_163588917.1	hypothetical protein	-
FEE39_RS09780	83984..84568	-	585	WP_163588918.1	hypothetical protein	-
FEE39_RS09785	84585..85190	-	606	WP_163588919.1	hypothetical protein	-
FEE39_RS09790	85174..85407	-	234	WP_163588920.1	hypothetical protein	-
FEE39_RS09795	85902..86174	+	273	WP_163588955.1	HU family DNA-binding protein	-
FEE39_RS09800	86308..86520	+	213	WP_163588921.1	hypothetical protein	-
FEE39_RS09805	86811..87092	-	282	WP_163588922.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FEE39_RS09810	87095..87310	-	216	WP_163588923.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..127062	127062

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-107)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6915.05 Da
• Isoelectric Point: 11.2377

>T127107 WP_163588915.1 NZ_CP040855:82766-82948 [Lactobacillus johnsonii]
MSSREVIKRLKADGWFLVRITGDHHHFKHPNRKGIVTVPHPNKDIPKGTLSSIYKQAGWK

Nucleotide

• Length: 183 bp

>T127107 NZ_CP040855:82766-82948 [Lactobacillus johnsonii]
ATGAGTTCACGTGAAGTCATAAAGAGGCTCAAGGCAGATGGATGGTTCTTGGTACGTATTACAGGTGACCATCATCATTT
CAAGCATCCTAATAGAAAAGGAATTGTTACTGTTCCTCATCCGAATAAGGATATCCCAAAAGGAACCTTGAGTAGTATTT
ATAAACAGGCGGGTTGGAAATAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 15331.41 Da
• Isoelectric Point: 4.6051

>AT127107 WP_163588916.1 NZ_CP040855:82981-83382 [Lactobacillus johnsonii]
MFYFATFHQNDQNQYEVNFPDLEPYAATYGDTLEEALQSAHDSLVGYLLTAEDFNETVPKPTIDPTQLNVKLNYPDFMMP
VEVNLKLEREKELNKLVKKTLTIPKYLNDLGKKNNINFSAILTNALKENLNLK

Nucleotide

• Length: 402 bp

>AT127107 NZ_CP040855:82981-83382 [Lactobacillus johnsonii]
ATGTTTTATTTTGCAACTTTTCATCAAAATGATCAAAATCAATATGAAGTTAATTTTCCGGATTTAGAACCATATGCAGC
TACATATGGTGATACTTTAGAAGAAGCGTTACAAAGTGCCCATGACTCTTTAGTAGGGTATCTACTAACTGCTGAGGATT
TTAATGAAACAGTTCCTAAACCAACTATTGATCCAACACAGTTAAATGTAAAGTTAAACTATCCAGATTTTATGATGCCT
GTAGAAGTAAATCTAAAATTAGAGAGAGAAAAAGAACTGAATAAGTTAGTAAAAAAGACCTTAACAATTCCAAAATATTT
AAACGATTTAGGTAAAAAAAATAATATTAACTTTTCAGCAATTCTTACAAATGCTTTAAAGGAAAATCTGAATCTTAAAT
AA