127104

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 146311..147419 Replicon plasmid p_unnamned1
Accession NZ_CP040850
Organism Enterococcus faecium strain F17E0263

Toxin (Protein)


Gene name MNTss Uniprot ID A0A0M4MHJ8
Locus tag FHK66_RS14580 Protein ID WP_002303392.1
Coordinates 146550..147419 (+) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID B9DSK1
Locus tag FHK66_RS14575 Protein ID WP_002303393.1
Coordinates 146311..146535 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FHK66_RS14555 141312..141875 + 564 Protein_155 recombinase zinc beta ribbon domain-containing protein -
FHK66_RS14560 142318..143802 + 1485 WP_002294513.1 ABC-F type ribosomal protection protein Lsa(E) -
FHK66_RS14565 143856..144659 + 804 WP_002294514.1 lincosamide nucleotidyltransferase Lnu(B) -
FHK66_RS14570 145347..146168 + 822 Protein_158 recombinase zinc beta ribbon domain-containing protein -
FHK66_RS14575 146311..146535 + 225 WP_002303393.1 helix-turn-helix transcriptional regulator Antitoxin
FHK66_RS14580 146550..147419 + 870 WP_002303392.1 nucleotidyltransferase domain-containing protein Toxin
FHK66_RS14585 147400..148134 + 735 WP_000662263.1 class I SAM-dependent methyltransferase -
FHK66_RS14590 148167..149030 + 864 WP_002294505.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
FHK66_RS14595 149074..149601 + 528 WP_002294507.1 adenine phosphoribosyltransferase -
FHK66_RS14600 149733..150542 + 810 WP_002294509.1 ANT(9) family aminoglycoside nucleotidyltransferase Spw -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32807.38 Da        Isoelectric Point: 4.7269

>T127104 WP_002303392.1 NZ_CP040850:146550-147419 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

>T127104 NZ_CP040850:146550-147419 [Enterococcus faecium]
ATGGTAGGTAATGTTATTCAAATAGTAACAGAGAAATTATCATCTTTGCCTTTTATAGAAGGCATTGTATTAGGGGGCTC
ACGTGCAAGAAGCACCCATACAGAAAATTCTGATATAGATATCGGAATCTATTACAATTCAGACTCATTTGACCTGACAG
CGATTAATCAAATTGCTACAGAGTTGGATGATGAGAATAGAAACAACCTTGTTGTACCTCCCGGAGCGTGGGGTGATTGG
GTTAATGGCGGTGGATGGTTAGTTATAAACGGGTGTCATGTTGACTTGATATTACGTGATATAAAACGGGTGGAACAAAT
AATCAAAGATACGGAGCAAGGAATTGTTACTGCCAATTATCAGACCGGGCATCCCCATGGTTATATAAGTGCTATGTATC
GTGGAGAATTAGCGATTAGTAAAATACTATATGCTAAGAACGAAAGCTTATGCGAATTAAAAAAGCAGGCAGAAATTTAC
CCTACTGCTCTAAAGAAAAGCTTGATGAACTTTTTTATATTTGAAGCTGAATTCTCTTTAATGTTTGTAAAAGCAAATGC
GGGGGCAGAGGATAAATATTATATTGCAGGCCATGTTTTTCGTATTATTTCATGCTTAAATCAAGTACTATTTGCATGTA
ATAATGCTTATTGCATTAACGAAAAGAAAGCTATAAAACTGCTTGAAACTTTTGAATATAAACCTGAAAAATATGCCGAA
AGAGTTAATCATATTTTTGAAGTACTCGGTCTTTCTCTTTTTGAATGCTACGACATGACCGAGAAACTTTATAAAGAAGT
GAATGAAATTGTATCGGAGATAAATAACTTTTTAAACGAGGAGAGTTCAGATGAAAGAAAACAAATATGA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8351.65 Da        Isoelectric Point: 4.5357

>AT127104 WP_002303393.1 NZ_CP040850:146311-146535 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGDEIKISNE

Download         Length: 225 bp

>AT127104 NZ_CP040850:146311-146535 [Enterococcus faecium]
ATGACTACGGCAGAAATGATTAAAGAACTGTGTGAGCAAATGAATATAAGTGTTTCCGAACTTGCTAGACGTATTGGCCA
GACTCCACAGAATTTCAATAAAAAATTACAACGAGAAACAGTAACCTTGGATGAGTTGAAGGCCATTGCTGATGTGCTAG
GTGTCAAGTTTGTGCAGGCATTTATTTTACCAGATGGAGATGAAATAAAAATATCTAACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MHJ8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M5SHM7

References