Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 1490563..1491134 Replicon chromosome
Accession NZ_CP040849
Organism Enterococcus faecium strain F17E0263

Toxin (Protein)


Gene name mazF Uniprot ID A0A828ZXV4
Locus tag FHK66_RS07765 Protein ID WP_002302307.1
Coordinates 1490563..1490904 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag FHK66_RS07770 Protein ID WP_002323011.1
Coordinates 1490904..1491134 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FHK66_RS07750 1486044..1487158 + 1115 WP_104776418.1 IS3 family transposase -
FHK66_RS07755 1488458..1489627 + 1170 WP_002300314.1 IS256 family transposase -
FHK66_RS07760 1489679..1490521 + 843 Protein_1456 IS982 family transposase -
FHK66_RS07765 1490563..1490904 - 342 WP_002302307.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FHK66_RS07770 1490904..1491134 - 231 WP_002323011.1 hypothetical protein Antitoxin
FHK66_RS07780 1491421..1491759 + 339 WP_002286804.1 hypothetical protein -
FHK66_RS07785 1491964..1492539 - 576 WP_002302305.1 SOS response-associated peptidase family protein -
FHK66_RS07800 1492903..1493484 - 582 WP_139424949.1 TetR/AcrR family transcriptional regulator -
FHK66_RS07805 1493677..1494303 - 627 WP_002300333.1 cysteine hydrolase -
FHK66_RS07810 1494325..1495653 - 1329 WP_180336731.1 FAD-containing oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13288.65 Da        Isoelectric Point: 9.9044

>T127102 WP_002302307.1 NZ_CP040849:c1490904-1490563 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIISQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T127102 NZ_CP040849:c1490904-1490563 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTTCACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT127102 WP_002323011.1 NZ_CP040849:c1491134-1490904 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT127102 NZ_CP040849:c1491134-1490904 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXV4

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References