Detailed information of TA system TA127100

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1367386..1367992
• Replicon: chromosome
• Accession: NZ_CP040846
• Organism: Thermococcus indicus strain IOH1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FH039_RS07540
• Protein ID: WP_139680818.1
• Coordinates: 1367594..1367992 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FH039_RS07535
• Protein ID: WP_139680817.1
• Coordinates: 1367386..1367607 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH039_RS07515 (FH039_07515)	1362994..1365282	-	2289	WP_139680814.1	alpha-amylase family glycosyl hydrolase	-
FH039_RS07520 (FH039_07520)	1365434..1365664	+	231	WP_014011940.1	LSm family protein	-
FH039_RS07525 (FH039_07525)	1365690..1365881	+	192	WP_139680815.1	50S ribosomal protein L37e	-
FH039_RS07530 (FH039_07530)	1365968..1367338	+	1371	WP_139680816.1	MATE family efflux transporter	-
FH039_RS07535 (FH039_07535)	1367386..1367607	+	222	WP_139680817.1	hypothetical protein	Antitoxin
FH039_RS07540 (FH039_07540)	1367594..1367992	+	399	WP_139680818.1	type II toxin-antitoxin system VapC family toxin	Toxin
FH039_RS07545 (FH039_07545)	1367989..1369083	-	1095	WP_139680819.1	VIT1/CCC1 transporter family protein	-
FH039_RS07550 (FH039_07550)	1369216..1370019	+	804	WP_139680820.1	PIG-L deacetylase family protein	-
FH039_RS07555 (FH039_07555)	1370012..1370461	-	450	WP_139680821.1	type II toxin-antitoxin system VapC family toxin	-
FH039_RS07560 (FH039_07560)	1370464..1370673	-	210	WP_139680822.1	hypothetical protein	-
FH039_RS07565 (FH039_07565)	1370670..1372124	-	1455	WP_139680823.1	beta-galactosidase BgaS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (3-129)

Antitoxin

• Putative antitoxin VapB3-like (1-73)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14875.19 Da
• Isoelectric Point: 4.9888

>T127100 WP_139680818.1 NZ_CP040846:1367594-1367992 [Thermococcus indicus]
VIVIDASALAKYVLLEPGWERVEELLFKDVVSLDYALAEVSNALWKHHVLYGEISREEFNKRSKVVDALPNVVVLESGMQ
YLEKSRGIAVNHRIPIYDALYIAQALKYGELATSDEKQGEIAKKLGVEVLYL

Nucleotide

• Length: 399 bp

>T127100 NZ_CP040846:1367594-1367992 [Thermococcus indicus]
GTGATAGTAATTGACGCCTCTGCACTTGCGAAGTACGTTCTCCTCGAACCCGGCTGGGAACGGGTAGAGGAGCTACTTTT
TAAAGACGTCGTCTCACTTGACTATGCCTTGGCAGAGGTTTCAAACGCCCTCTGGAAACATCACGTCCTCTACGGTGAGA
TATCCCGGGAGGAGTTCAATAAGAGATCAAAGGTTGTGGATGCTCTCCCGAACGTCGTCGTTCTTGAGAGTGGAATGCAG
TATTTGGAGAAATCAAGGGGGATAGCTGTTAACCACCGAATCCCAATCTATGACGCCCTCTACATCGCTCAGGCCCTGAA
GTATGGCGAACTTGCCACGAGCGACGAAAAGCAGGGAGAAATAGCGAAAAAACTCGGCGTTGAGGTGCTCTACCTCTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8623.93 Da
• Isoelectric Point: 8.5278

>AT127100 WP_139680817.1 NZ_CP040846:1367386-1367607 [Thermococcus indicus]
MAVISVRVPDELKEKMKKYDINWSEEIRRFIALRISKEEKKRTLEMMHELLAGGTPAEEGTAKRYVREDRDSN

Nucleotide

• Length: 222 bp

>AT127100 NZ_CP040846:1367386-1367607 [Thermococcus indicus]
ATGGCGGTGATAAGCGTCCGCGTTCCGGACGAGCTAAAGGAGAAGATGAAGAAGTACGACATCAACTGGAGCGAGGAGAT
AAGGCGGTTCATAGCCTTGAGGATAAGCAAAGAGGAAAAGAAAAGAACCCTTGAGATGATGCACGAACTGCTTGCCGGAG
GAACCCCCGCCGAGGAAGGGACTGCAAAAAGATACGTGAGGGAGGATCGTGATAGTAATTGA