Detailed information of TA system TA127098

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 67917..68488
• Replicon: plasmid pD13
• Accession: NZ_CP040845
• Organism: Leptospira weilii strain CUD13

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FHG68_RS21150
• Protein ID: WP_016758353.1
• Coordinates: 67917..68258 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A828Z2G4
• Locus tag: FHG68_RS21155
• Protein ID: WP_004499620.1
• Coordinates: 68252..68488 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FHG68_RS21120	64579..65019	+	441	WP_004497511.1	winged helix-turn-helix domain-containing protein	-
FHG68_RS21125	65075..65578	+	504	WP_080634873.1	IS630 family transposase	-
FHG68_RS21130	65701..66102	-	402	WP_004500217.1	helix-turn-helix domain-containing protein	-
FHG68_RS21135	66104..66391	-	288	WP_026054191.1	type II toxin-antitoxin system HigB family toxin	-
FHG68_RS21140	66536..66913	-	378	WP_036075665.1	hypothetical protein	-
FHG68_RS21730	67134..67415	-	282	WP_004497523.1	hypothetical protein	-
FHG68_RS21145	67658..67873	-	216	WP_004497509.1	hypothetical protein	-
FHG68_RS21150	67917..68258	-	342	WP_016758353.1	endoribonuclease MazF	Toxin
FHG68_RS21155	68252..68488	-	237	WP_004499620.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FHG68_RS21160	68828..69217	-	390	WP_036075668.1	hypothetical protein	-
FHG68_RS21165	69258..70330	-	1073	Protein_65	IS3 family transposase	-
FHG68_RS21175	70389..70523	+	135	WP_085988574.1	AraC family transcriptional regulator	-
FHG68_RS21180	70719..70949	-	231	WP_004497519.1	hypothetical protein	-
FHG68_RS21185	71109..71905	-	797	Protein_68	IS5 family transposase	-
FHG68_RS21190	72072..72275	-	204	WP_004497508.1	hypothetical protein	-
FHG68_RS21200	72651..73289	-	639	WP_004499330.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..95487	95487

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (11-111)

Antitoxin

• SpoVT-AbrB domain (6-48)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.17 Da
• Isoelectric Point: 10.1024

>T127098 WP_016758353.1 NZ_CP040845:c68258-67917 [Leptospira weilii]
MVKSRKYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVTIKLKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLVF

Nucleotide

• Length: 342 bp

>T127098 NZ_CP040845:c68258-67917 [Leptospira weilii]
TTGGTAAAGAGTAGAAAATATACTCCTGAAAAAGGAGATATAGTTTGGCTGAACTTTACCCCGCAAGCAGGTCATGAACA
AAAAGGTCGTAGACCAGCTTTAGTTTTGTCACCTAAAGAATATAATTCGAAAACTGGATTAGCAATTTTCTGTCCGATTA
CTAGTAAGATTAAAGGTTATCCATTCGAGGTAACAATTAAATTGAAGAAAATTGATGGTGTAATTCTTTCTGATCAAGTG
AAAAACTTAGACTGGACTATTCGTGAAGCTGAATTTATAGAATCAATAAATAAAGTTTCTTTGAAAGAAGTTTTAGATAA
TATTAAGTTATTAGTATTTTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8756.06 Da
• Isoelectric Point: 7.2016

>AT127098 WP_004499620.1 NZ_CP040845:c68488-68252 [Leptospira weilii]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGNKIVIYPMKKASLEEKLSKITKQNLHSEISTGNSIGKEAW

Nucleotide

• Length: 237 bp

>AT127098 NZ_CP040845:c68488-68252 [Leptospira weilii]
ATGGAATCAATTATTCAAAAATGGGGTAATAGCTTAGGAATTAGAATTCCAAAAGCAATGGCAACGGAATTAGAGTTAAA
TGACGGAAGCCACGTTGAATTACAATATGAAGGGAATAAGATTGTAATATATCCAATGAAAAAAGCTTCATTAGAAGAAA
AACTTTCTAAAATTACGAAACAAAATCTTCACTCTGAAATATCAACTGGTAACTCAATAGGTAAAGAAGCTTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A828Z2G4