Detailed information of TA system TA127097

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 817954..818582
• Replicon: chromosome
• Accession: NZ_CP040843
• Organism: Leptospira weilii strain CUD13

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: M6PYD2
• Locus tag: FHG68_RS03930
• Protein ID: WP_004495849.1
• Coordinates: 818181..818582 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FHG68_RS03925
• Protein ID: WP_016759326.1
• Coordinates: 817954..818184 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FHG68_RS03875	813010..813702	+	693	WP_004496073.1	cytochrome c biogenesis protein CcsA	-
FHG68_RS03880	813709..814839	+	1131	WP_004499160.1	SpoIIE family protein phosphatase	-
FHG68_RS03885	814836..815702	+	867	WP_016759636.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
FHG68_RS03890	815678..815926	+	249	WP_002614445.1	phosphoribosylformylglycinamidine synthase subunit PurS	-
FHG68_RS03895	815923..816582	+	660	WP_002614451.1	phosphoribosylformylglycinamidine synthase subunit PurQ	-
FHG68_RS03900	816699..816917	-	219	WP_004499270.1	IS982 family transposase	-
FHG68_RS03905	816918..817024	-	107	Protein_704	IS982 family transposase	-
FHG68_RS21415	817126..817709	-	584	Protein_705	IS982 family transposase	-
FHG68_RS03925	817954..818184	+	231	WP_016759326.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FHG68_RS03930	818181..818582	+	402	WP_004495849.1	type II toxin-antitoxin system VapC family toxin	Toxin
FHG68_RS03935	818868..820025	+	1158	WP_036075452.1	hypothetical protein	-
FHG68_RS03940	820029..820859	+	831	WP_004501498.1	hypothetical protein	-
FHG68_RS03945	821013..821537	+	525	WP_004499241.1	hypothetical protein	-
FHG68_RS03955	822296..823015	+	720	WP_002616817.1	flagellar filament outer layer protein Flaa	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• SpoVT-AbrB domain (7-46)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 15600.27 Da
• Isoelectric Point: 9.2916

>T127097 WP_004495849.1 NZ_CP040843:818181-818582 [Leptospira weilii]
MTQYLLDTNICIYIINQKPESVYKKFKKIKLENIFISSITEFELKYGVQKSLHFERNLKVLEEFLSYLNILPFVSEDTNK
AAKVRVELERVGKPIGPFDLLIASQALSNKLTLVTNNEKEFTRIKEIKIENWL

Nucleotide

• Length: 402 bp

>T127097 NZ_CP040843:818181-818582 [Leptospira weilii]
ATGACTCAATACTTGTTGGATACGAATATCTGTATCTATATAATTAATCAAAAGCCGGAATCTGTTTATAAGAAATTTAA
AAAGATTAAATTAGAGAATATTTTCATTTCATCAATTACGGAATTTGAATTAAAATACGGTGTTCAGAAGAGTCTTCATT
TTGAAAGAAATCTGAAAGTCTTAGAAGAATTTTTAAGTTATTTGAATATTCTTCCGTTTGTTAGTGAAGATACGAATAAA
GCTGCAAAAGTTCGAGTAGAATTAGAAAGAGTAGGGAAACCAATTGGTCCTTTCGATCTTCTAATCGCTTCTCAGGCATT
ATCGAATAAACTTACGCTTGTAACAAACAATGAAAAAGAATTTACTCGGATTAAAGAAATTAAAATTGAAAACTGGCTTT
AA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8975.12 Da
• Isoelectric Point: 9.8802

>AT127097 WP_016759326.1 NZ_CP040843:817954-818184 [Leptospira weilii]
MNKAKIFKNGDSQAIRLPKDYRFKGKEVYIRKDGDNVILTPIDDAVDRFWNTVNNFSEDLLIDRNQPNGYDKRNRI

Nucleotide

• Length: 231 bp

>AT127097 NZ_CP040843:817954-818184 [Leptospira weilii]
ATGAATAAAGCTAAAATTTTTAAAAATGGCGACAGCCAAGCAATACGTTTACCAAAGGACTATCGTTTTAAGGGTAAAGA
AGTTTATATACGAAAAGATGGCGATAACGTCATTTTAACGCCAATAGACGATGCGGTTGATCGATTTTGGAATACGGTTA
ATAATTTTTCTGAAGATCTGCTTATTGATCGGAATCAACCAAATGGCTACGATAAACGAAATAGAATATGA

Structures

Toxin

• AlphaFold DB: A0A828Z772