Detailed information of TA system TA127095

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 61666..62237
• Replicon: plasmid pDO6
• Accession: NZ_CP040842
• Organism: Leptospira weilii strain CUDO6

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FHG67_RS21365
• Protein ID: WP_016758353.1
• Coordinates: 61666..62007 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A828Z2G4
• Locus tag: FHG67_RS21370
• Protein ID: WP_004499620.1
• Coordinates: 62001..62237 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FHG67_RS21335	58328..58768	+	441	WP_004497511.1	winged helix-turn-helix domain-containing protein	-
FHG67_RS21340	58824..59327	+	504	WP_080634873.1	IS630 family transposase	-
FHG67_RS21345	59450..59851	-	402	WP_004500217.1	helix-turn-helix domain-containing protein	-
FHG67_RS21350	59853..60140	-	288	WP_026054191.1	type II toxin-antitoxin system HigB family toxin	-
FHG67_RS21355	60285..60662	-	378	WP_036075665.1	hypothetical protein	-
FHG67_RS21965	60883..61164	-	282	WP_004497523.1	hypothetical protein	-
FHG67_RS21360	61407..61622	-	216	WP_004497509.1	hypothetical protein	-
FHG67_RS21365	61666..62007	-	342	WP_016758353.1	endoribonuclease MazF	Toxin
FHG67_RS21370	62001..62237	-	237	WP_004499620.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FHG67_RS21375	62577..62966	-	390	WP_036075668.1	hypothetical protein	-
FHG67_RS21380	63007..64079	-	1073	Protein_59	IS3 family transposase	-
FHG67_RS21395	64468..64698	-	231	WP_004497519.1	hypothetical protein	-
FHG67_RS21400	64858..65654	-	797	Protein_61	IS5 family transposase	-
FHG67_RS21405	65821..66024	-	204	WP_004497508.1	hypothetical protein	-
FHG67_RS21415	66400..67038	-	639	WP_004499330.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..89236	89236

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (11-111)

Antitoxin

• SpoVT-AbrB domain (6-48)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.17 Da
• Isoelectric Point: 10.1024

>T127095 WP_016758353.1 NZ_CP040842:c62007-61666 [Leptospira weilii]
MVKSRKYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVTIKLKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLVF

Nucleotide

• Length: 342 bp

>T127095 NZ_CP040842:c62007-61666 [Leptospira weilii]
TTGGTAAAGAGTAGAAAATATACTCCTGAAAAAGGAGATATAGTTTGGCTGAACTTTACCCCGCAAGCAGGTCATGAACA
AAAAGGTCGTAGACCAGCTTTAGTTTTGTCACCTAAAGAATATAATTCGAAAACTGGATTAGCAATTTTCTGTCCGATTA
CTAGTAAGATTAAAGGTTATCCATTCGAGGTAACAATTAAATTGAAGAAAATTGATGGTGTAATTCTTTCTGATCAAGTG
AAAAACTTAGACTGGACTATTCGTGAAGCTGAATTTATAGAATCAATAAATAAAGTTTCTTTGAAAGAAGTTTTAGATAA
TATTAAGTTATTAGTATTTTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8756.06 Da
• Isoelectric Point: 7.2016

>AT127095 WP_004499620.1 NZ_CP040842:c62237-62001 [Leptospira weilii]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGNKIVIYPMKKASLEEKLSKITKQNLHSEISTGNSIGKEAW

Nucleotide

• Length: 237 bp

>AT127095 NZ_CP040842:c62237-62001 [Leptospira weilii]
ATGGAATCAATTATTCAAAAATGGGGTAATAGCTTAGGAATTAGAATTCCAAAAGCAATGGCAACGGAATTAGAGTTAAA
TGACGGAAGCCACGTTGAATTACAATATGAAGGGAATAAGATTGTAATATATCCAATGAAAAAAGCTTCATTAGAAGAAA
AACTTTCTAAAATTACGAAACAAAATCTTCACTCTGAAATATCAACTGGTAACTCAATAGGTAAAGAAGCTTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A828Z2G4