Detailed information of TA system TA127093

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpIK/PemK(toxin)
• Location: 758628..759204
• Replicon: chromosome
• Accession: NZ_CP040840
• Organism: Leptospira weilii strain CUDO6

Toxin (Protein)

• Gene name: chpK
• Uniprot ID: -
• Locus tag: FHG67_RS03615
• Protein ID: WP_016758518.1
• Coordinates: 758863..759204 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: chpI
• Uniprot ID: M6QGN1
• Locus tag: FHG67_RS03610
• Protein ID: WP_004495460.1
• Coordinates: 758628..758876 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FHG67_RS03590	754908..756101	-	1194	WP_004499273.1	M23 family metallopeptidase	-
FHG67_RS03600	757767..758039	+	273	WP_036075712.1	transposase	-
FHG67_RS21630	758036..758197	+	162	WP_004499288.1	transposase	-
FHG67_RS03605	758229..758492	+	264	WP_142499899.1	IS3 family transposase	-
FHG67_RS03610	758628..758876	+	249	WP_004495460.1	hypothetical protein	Antitoxin
FHG67_RS03615	758863..759204	+	342	WP_016758518.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FHG67_RS21635	759481..759750	+	270	WP_036075696.1	hypothetical protein	-
FHG67_RS03625	759740..760162	+	423	WP_004499259.1	PIN domain-containing protein	-
FHG67_RS03630	760493..761086	+	594	WP_004501734.1	dihydrofolate reductase family protein	-
FHG67_RS03635	761393..762592	-	1200	WP_004501723.1	membrane protein	-
FHG67_RS03640	762596..763300	-	705	WP_004501733.1	glycosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12451.35 Da
• Isoelectric Point: 8.6144

>T127093 WP_016758518.1 NZ_CP040840:758863-759204 [Leptospira weilii]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSNINTIVSIAITSNLNLSEAPGNVLISKKDSNLSKDSVVNVSQIV
TLDKKRFLNKVGKLKSNKLNEVEAGLKLVTDLD

Nucleotide

• Length: 342 bp

>T127093 NZ_CP040840:758863-759204 [Leptospira weilii]
ATGATTCGTGGTGAAATTTGGTGGGTAGACTTGGGAATTCCCTTTGGAAGTGAGCCAGGATTCAAACGTCCGGTTTTGAT
AATTCAAGATGACTCTTTTAATCAAAGTAATATTAATACAATAGTTTCGATTGCAATTACATCGAATTTAAATCTATCCG
AAGCACCAGGTAACGTTTTAATTAGTAAGAAAGATTCGAATTTATCAAAGGATTCAGTCGTAAATGTCTCTCAAATTGTG
ACTTTAGATAAAAAAAGATTTCTTAATAAAGTAGGAAAACTTAAATCCAATAAACTAAATGAAGTTGAAGCGGGGCTAAA
GTTAGTTACAGACTTAGATTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9287.59 Da
• Isoelectric Point: 10.1669

>AT127093 WP_004495460.1 NZ_CP040840:758628-758876 [Leptospira weilii]
MKTAISIPDELFKTAEKTAKKLGIPRSQLFAKALEEFIQSHSKESVTEKLNKVYNSKSSETKNNITDLSVESLRKSLKND
SW

Nucleotide

• Length: 249 bp

>AT127093 NZ_CP040840:758628-758876 [Leptospira weilii]
ATGAAAACGGCAATCTCAATTCCTGATGAGCTATTTAAGACCGCAGAAAAAACAGCTAAAAAGCTTGGTATTCCTCGTAG
TCAGTTATTTGCAAAAGCATTAGAGGAATTTATTCAATCTCATAGTAAAGAATCCGTAACTGAGAAATTAAATAAAGTCT
ACAATAGTAAATCTAGCGAAACTAAAAATAACATCACTGACTTATCCGTAGAATCACTTCGCAAAAGTTTAAAAAATGAT
TCGTGGTGA

Structures

Antitoxin

• AlphaFold DB: M6QGN1