Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 88415..89058 Replicon plasmid pSH12_KPC
Accession NZ_CP040835
Organism Klebsiella pneumoniae strain SH12

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag FHY43_RS28805 Protein ID WP_001044770.1
Coordinates 88642..89058 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag FHY43_RS28800 Protein ID WP_001261282.1
Coordinates 88415..88645 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FHY43_RS28765 (83638) 83638..84417 - 780 WP_013214009.1 site-specific integrase -
FHY43_RS28770 (84601) 84601..85605 - 1005 WP_011977814.1 hypothetical protein -
FHY43_RS28775 (85635) 85635..85838 - 204 WP_011977813.1 hypothetical protein -
FHY43_RS28780 (85884) 85884..86405 - 522 WP_013214008.1 hypothetical protein -
FHY43_RS28785 (86463) 86463..86855 - 393 WP_011977811.1 hypothetical protein -
FHY43_RS28790 (86892) 86892..87836 - 945 WP_011977810.1 hypothetical protein -
FHY43_RS28795 (87997) 87997..88458 - 462 WP_014343465.1 hypothetical protein -
FHY43_RS28800 (88415) 88415..88645 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
FHY43_RS28805 (88642) 88642..89058 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
FHY43_RS28810 (89132) 89132..90694 + 1563 WP_004206609.1 AAA family ATPase -
FHY43_RS28815 (90679) 90679..91701 + 1023 WP_000361404.1 helicase UvrD -
FHY43_RS28820 (91957) 91957..92654 + 698 WP_223174630.1 IS1 family transposase -
FHY43_RS28825 (92683) 92683..92955 + 273 Protein_113 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2 / catA2 - 1..167468 167468


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T127089 WP_001044770.1 NZ_CP040835:88642-89058 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T127089 NZ_CP040835:88642-89058 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT127089 WP_001261282.1 NZ_CP040835:88415-88645 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT127089 NZ_CP040835:88415-88645 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References