Detailed information of TA system TA127085

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 205682..206204
• Replicon: plasmid pSH12_Vir
• Accession: NZ_CP040834
• Organism: Klebsiella pneumoniae strain SH12

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A2J4R0S6
• Locus tag: FHY43_RS27900
• Protein ID: WP_004181778.1
• Coordinates: 205920..206204 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4R0U8
• Locus tag: FHY43_RS27895
• Protein ID: WP_004181777.1
• Coordinates: 205682..205930 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FHY43_RS27875 (FHY43_28295)	200891..201712	-	822	WP_004181772.1	hypothetical protein	-
FHY43_RS27880 (FHY43_28300)	201774..202127	-	354	WP_004181774.1	hypothetical protein	-
FHY43_RS27885 (FHY43_28305)	202272..203258	-	987	WP_101992392.1	hypothetical protein	-
FHY43_RS27890 (FHY43_28310)	203592..205391	-	1800	WP_202153275.1	ATP-dependent helicase	-
FHY43_RS27895 (FHY43_28315)	205682..205930	+	249	WP_004181777.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FHY43_RS27900 (FHY43_28320)	205920..206204	+	285	WP_004181778.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FHY43_RS27905 (FHY43_28325)	206221..206322	-	102	Protein_232	IS200/IS605 family transposase	-
FHY43_RS27910 (FHY43_28330)	206358..207584	+	1227	WP_040209639.1	RNA-guided endonuclease TnpB family protein	-
FHY43_RS27915 (FHY43_28335)	207855..208079	-	225	Protein_234	transposase	-
FHY43_RS27920 (FHY43_28340)	208158..208586	-	429	WP_077257669.1	IS200/IS605 family transposase	-
FHY43_RS27925 (FHY43_28345)	208622..209809	+	1188	WP_040209642.1	RNA-guided endonuclease TnpB family protein	-
FHY43_RS29725	209854..210225	-	372	WP_040209644.1	hypothetical protein	-
FHY43_RS29730	210222..210566	-	345	WP_228306996.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1B / aac(3)-IId / blaSHV-12 / qnrB4 / blaDHA-1 / sul1 / armA / msr(E) / mph(E) / mph(A)	rmpA / iutA / iucD / iucC / iucB / iucA	1..284671	284671

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10970.69 Da
• Isoelectric Point: 10.6516

>T127085 WP_004181778.1 NZ_CP040834:205920-206204 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Nucleotide

• Length: 285 bp

>T127085 NZ_CP040834:205920-206204 [Klebsiella pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTAACCAAACACAGAAATTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8925.17 Da
• Isoelectric Point: 3.8209

>AT127085 WP_004181777.1 NZ_CP040834:205682-205930 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Nucleotide

• Length: 249 bp

>AT127085 NZ_CP040834:205682-205930 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCTGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Structures

Toxin

• AlphaFold DB: A0A2J4R0S6

Antitoxin

• AlphaFold DB: A0A2J4R0U8