Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 93170..93840 | Replicon | plasmid pSH12_Vir |
| Accession | NZ_CP040834 | ||
| Organism | Klebsiella pneumoniae strain SH12 | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | Q6U619 |
| Locus tag | FHY43_RS27315 | Protein ID | WP_004213072.1 |
| Coordinates | 93397..93840 (+) | Length | 148 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | Q6U620 |
| Locus tag | FHY43_RS27310 | Protein ID | WP_004213073.1 |
| Coordinates | 93170..93400 (+) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| FHY43_RS27275 (FHY43_27615) | 88345..89097 | - | 753 | Protein_104 | replication initiation protein RepM | - |
| FHY43_RS27280 (FHY43_27620) | 89163..89867 | + | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| FHY43_RS27285 (FHY43_27625) | 89813..90292 | - | 480 | WP_228750710.1 | hypothetical protein | - |
| FHY43_RS27290 (FHY43_27630) | 90282..90572 | - | 291 | WP_004213078.1 | hypothetical protein | - |
| FHY43_RS27295 (FHY43_27635) | 90924..91130 | + | 207 | WP_004213077.1 | hypothetical protein | - |
| FHY43_RS27300 (FHY43_27640) | 91120..91413 | - | 294 | WP_004213076.1 | hypothetical protein | - |
| FHY43_RS27305 (FHY43_27645) | 91429..92562 | - | 1134 | WP_004213075.1 | DUF3800 domain-containing protein | - |
| FHY43_RS27310 (FHY43_27650) | 93170..93400 | + | 231 | WP_004213073.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin |
| FHY43_RS27315 (FHY43_27655) | 93397..93840 | + | 444 | WP_004213072.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| FHY43_RS29705 | 93989..94240 | + | 252 | WP_186987481.1 | hypothetical protein | - |
| FHY43_RS27325 (FHY43_27665) | 94263..94567 | - | 305 | Protein_114 | transposase | - |
| FHY43_RS27330 | 94984..95618 | + | 635 | Protein_115 | mucoid phenotype regulator RmpA2 | - |
| FHY43_RS27335 (FHY43_27685) | 96136..96539 | - | 404 | Protein_116 | GAF domain-containing protein | - |
| FHY43_RS27340 (FHY43_27695) | 96630..97550 | - | 921 | WP_004213934.1 | DUF1471 domain-containing protein | - |
| FHY43_RS27345 (FHY43_27700) | 97599..98090 | - | 492 | WP_011251327.1 | DM13 domain-containing protein | - |
| FHY43_RS27350 (FHY43_27705) | 98153..98428 | - | 276 | WP_004213932.1 | carboxymuconolactone decarboxylase family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | blaTEM-1B / aac(3)-IId / blaSHV-12 / qnrB4 / blaDHA-1 / sul1 / armA / msr(E) / mph(E) / mph(A) | rmpA / iutA / iucD / iucC / iucB / iucA | 1..284671 | 284671 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 148 a.a. Molecular weight: 16006.74 Da Isoelectric Point: 9.3642
>T127083 WP_004213072.1 NZ_CP040834:93397-93840 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS
Download Length: 444 bp
>T127083 NZ_CP040834:93397-93840 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8677.73 Da Isoelectric Point: 4.4563
>AT127083 WP_004213073.1 NZ_CP040834:93170-93400 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP
Download Length: 231 bp
>AT127083 NZ_CP040834:93170-93400 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|