Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5367394..5368019 Replicon chromosome
Accession NZ_CP040833
Organism Klebsiella pneumoniae strain SH12

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag FHY43_RS26390 Protein ID WP_002882817.1
Coordinates 5367394..5367777 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag FHY43_RS26395 Protein ID WP_004150355.1
Coordinates 5367777..5368019 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FHY43_RS26375 (FHY43_26620) 5364760..5365662 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
FHY43_RS26380 (FHY43_26625) 5365659..5366294 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
FHY43_RS26385 (FHY43_26630) 5366291..5367220 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
FHY43_RS26390 (FHY43_26635) 5367394..5367777 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
FHY43_RS26395 (FHY43_26640) 5367777..5368019 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
FHY43_RS26400 (FHY43_26645) 5368224..5369141 + 918 WP_002882812.1 alpha/beta hydrolase -
FHY43_RS26405 (FHY43_26650) 5369155..5370096 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
FHY43_RS26410 (FHY43_26655) 5370141..5370578 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
FHY43_RS26415 (FHY43_26660) 5370575..5371435 - 861 WP_002882807.1 virulence factor BrkB family protein -
FHY43_RS26420 (FHY43_26665) 5371429..5372028 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T127081 WP_002882817.1 NZ_CP040833:c5367777-5367394 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T127081 NZ_CP040833:c5367777-5367394 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT127081 WP_004150355.1 NZ_CP040833:c5368019-5367777 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT127081 NZ_CP040833:c5368019-5367777 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References