Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4170066..4170685 Replicon chromosome
Accession NZ_CP040833
Organism Klebsiella pneumoniae strain SH12

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag FHY43_RS20645 Protein ID WP_002892050.1
Coordinates 4170467..4170685 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag FHY43_RS20640 Protein ID WP_002892066.1
Coordinates 4170066..4170440 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FHY43_RS20630 (FHY43_20855) 4165218..4166411 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
FHY43_RS20635 (FHY43_20860) 4166434..4169580 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
FHY43_RS20640 (FHY43_20865) 4170066..4170440 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
FHY43_RS20645 (FHY43_20870) 4170467..4170685 + 219 WP_002892050.1 HHA domain-containing protein Toxin
FHY43_RS20650 (FHY43_20875) 4170844..4171410 + 567 WP_002892030.1 maltose O-acetyltransferase -
FHY43_RS20655 4171382..4171522 - 141 WP_004147370.1 hypothetical protein -
FHY43_RS20660 (FHY43_20880) 4171543..4172013 + 471 WP_002892026.1 YlaC family protein -
FHY43_RS20665 (FHY43_20885) 4171988..4173439 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
FHY43_RS20670 (FHY43_20890) 4173540..4174238 + 699 WP_002892021.1 GNAT family protein -
FHY43_RS20675 (FHY43_20895) 4174235..4174375 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
FHY43_RS20680 (FHY43_20900) 4174375..4174638 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T127077 WP_002892050.1 NZ_CP040833:4170467-4170685 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T127077 NZ_CP040833:4170467-4170685 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT127077 WP_002892066.1 NZ_CP040833:4170066-4170440 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT127077 NZ_CP040833:4170066-4170440 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References