Detailed information of TA system TA127063

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 343584..344192
• Replicon: chromosome
• Accession: NZ_CP040829
• Organism: Paenibacillus polymyxa strain ZF129

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: H6CFY1
• Locus tag: FGY93_RS01680
• Protein ID: WP_007429335.1
• Coordinates: 343584..343934 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: FGY93_RS01685
• Protein ID: WP_170970806.1
• Coordinates: 343941..344192 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGY93_RS01650	339095..339970	+	876	WP_126666681.1	Cof-type HAD-IIB family hydrolase	-
FGY93_RS01665	340287..340799	-	513	WP_013369929.1	SprT family protein	-
FGY93_RS01670	340943..341077	+	135	WP_013369928.1	cortex morphogenetic protein CmpA	-
FGY93_RS01675	341183..343405	-	2223	WP_180361594.1	RNA-binding transcriptional accessory protein	-
FGY93_RS01680	343584..343934	-	351	WP_007429335.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FGY93_RS01685	343941..344192	-	252	WP_170970806.1	ribbon-helix-helix protein, CopG family	Antitoxin
FGY93_RS01690	344516..345712	-	1197	WP_013369924.1	alanine racemase	-
FGY93_RS01695	345981..347258	-	1278	WP_126666682.1	outer membrane lipoprotein carrier protein LolA	-
FGY93_RS01700	347369..348001	-	633	WP_126666683.1	LysE family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12822.77 Da
• Isoelectric Point: 5.6195

>T127063 WP_007429335.1 NZ_CP040829:c343934-343584 [Paenibacillus polymyxa]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAATHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDEETMRKVSDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T127063 NZ_CP040829:c343934-343584 [Paenibacillus polymyxa]
TTGATCGTAAAGCGCGGCGACGTTTTTTTTGCAGACCTTTCACCCGTAGTCGGTTCCGAGCAAGGTGGCGTCAGACCTGT
GCTGATCATTCAAAATGATATCGGCAACCGGTTTAGCCCTACCGTGATCGTAGCAGCCATCACTGCACAGATTCAGAAGG
CAAAGCTGCCTACACACGTGGAAATCGACGCGGCGACGCATGGCTTTGATCGTGATTCTGTTGTTCTTCTGGAGCAAATC
CGGACAATCGACAAGCAAAGGCTTACCGACAAAATCACCCATTTGGATGAAGAAACCATGCGCAAAGTGAGCGACTCGCT
ACAGATCAGTCTCGGTTTGATTGATTTTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9465.86 Da
• Isoelectric Point: 6.4671

>AT127063 WP_170970806.1 NZ_CP040829:c344192-343941 [Paenibacillus polymyxa]
MISLPDHLLQEVDGIVAMENSNRSEFIRQAMKLYVGERKKRYIREAMQRGYMEMAKINLTMASEAFHAEEDANSTLGRTV
SGV

Nucleotide

• Length: 252 bp

>AT127063 NZ_CP040829:c344192-343941 [Paenibacillus polymyxa]
ATGATCAGTTTGCCAGATCATCTCTTGCAGGAAGTGGACGGGATCGTAGCTATGGAAAATTCCAACCGCAGTGAATTTAT
CAGGCAGGCAATGAAGCTTTATGTAGGTGAACGTAAAAAACGTTACATTCGTGAAGCTATGCAGCGTGGATACATGGAAA
TGGCTAAAATTAACCTGACCATGGCATCCGAAGCCTTTCACGCGGAGGAAGATGCAAACAGCACCTTGGGCCGTACAGTT
AGCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: H6CFY1