Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1957091..1957716 Replicon chromosome
Accession NZ_CP040817
Organism Dickeya zeae strain PL65

Toxin (Protein)


Gene name Hha Uniprot ID E0SD87
Locus tag FGI21_RS08580 Protein ID WP_009114000.1
Coordinates 1957513..1957716 (+) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID D2BUK3
Locus tag FGI21_RS08575 Protein ID WP_012883774.1
Coordinates 1957091..1957459 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FGI21_RS08560 (FGI21_08610) 1952851..1955997 + 3147 WP_168363200.1 efflux RND transporter permease subunit -
FGI21_RS08565 (FGI21_08615) 1956171..1956422 + 252 WP_168363201.1 type B 50S ribosomal protein L31 -
FGI21_RS08570 (FGI21_08620) 1956438..1956581 + 144 WP_012770724.1 type B 50S ribosomal protein L36 -
FGI21_RS08575 (FGI21_08625) 1957091..1957459 + 369 WP_012883774.1 Hha toxicity modulator TomB Antitoxin
FGI21_RS08580 (FGI21_08630) 1957513..1957716 + 204 WP_009114000.1 HHA domain-containing protein Toxin
FGI21_RS08590 (FGI21_08640) 1958267..1958593 + 327 WP_220178200.1 MGMT family protein -
FGI21_RS08595 (FGI21_08645) 1958634..1959215 - 582 WP_220178201.1 YbaY family lipoprotein -
FGI21_RS08600 (FGI21_08650) 1959433..1960296 + 864 WP_220178202.1 acyl-CoA thioesterase II -
FGI21_RS08605 (FGI21_08655) 1960420..1961712 - 1293 WP_168363204.1 ammonium transporter AmtB -
FGI21_RS08610 (FGI21_08660) 1961756..1962094 - 339 WP_002208627.1 P-II family nitrogen regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8133.51 Da        Isoelectric Point: 8.8500

>T127058 WP_009114000.1 NZ_CP040817:1957513-1957716 [Dickeya zeae]
MKKIDYLMRLRKCTTIDTLERVIEKNKYELSNDELEMFYSAADHRLAELTMNKLYDKVPTAVWKYVR

Download         Length: 204 bp

>T127058 NZ_CP040817:1957513-1957716 [Dickeya zeae]
ATGAAAAAAATCGACTATTTAATGCGTTTGCGTAAATGTACAACCATCGACACGCTTGAGCGTGTTATAGAAAAAAACAA
GTACGAGCTTTCTAACGACGAACTTGAAATGTTCTATTCAGCTGCAGACCATCGTCTGGCTGAGCTAACGATGAATAAAC
TTTACGACAAAGTGCCAACTGCCGTGTGGAAATACGTTCGATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14186.04 Da        Isoelectric Point: 5.1811

>AT127058 WP_012883774.1 NZ_CP040817:1957091-1957459 [Dickeya zeae]
MDEYTPQHYDIAQLRFLCENLHDESIATLGDSSRGWVNDPTSAVNLQLNELIEHIAAFVVTYKIKYPHEAALCERVEKYL
DDTYILFSNYGINDSELQKWKKSKSQLFRMFSEKSVCTVVKT

Download         Length: 369 bp

>AT127058 NZ_CP040817:1957091-1957459 [Dickeya zeae]
ATGGATGAGTACACACCTCAACATTATGATATTGCCCAACTCAGATTCTTGTGTGAAAATCTGCACGACGAAAGTATCGC
AACATTAGGTGACAGCAGTCGTGGCTGGGTGAATGATCCGACCTCTGCGGTCAATCTTCAACTAAACGAACTTATCGAAC
ACATTGCGGCATTTGTTGTTACCTACAAAATAAAATATCCGCATGAAGCGGCGTTGTGTGAGCGGGTTGAAAAATACCTG
GACGATACTTATATTCTTTTCAGCAACTACGGTATAAATGATTCAGAGTTGCAGAAATGGAAAAAATCCAAGTCGCAATT
ATTCAGAATGTTCTCAGAAAAGAGCGTCTGTACGGTAGTAAAAACTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1X3RNQ0

Antitoxin

Source ID Structure
AlphaFold DB A0A2K9QCC2

References