Detailed information of TA system TA127055

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1063351..1063968
• Replicon: chromosome
• Accession: NZ_CP040817
• Organism: Dickeya zeae strain PL65

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: C6CDL0
• Locus tag: FGI21_RS04800
• Protein ID: WP_012764944.1
• Coordinates: 1063789..1063968 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: FGI21_RS04795
• Protein ID: WP_220177896.1
• Coordinates: 1063351..1063761 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGI21_RS04780 (FGI21_04785)	1059127..1059966	-	840	WP_038904570.1	GPO family capsid scaffolding protein	-
FGI21_RS04785 (FGI21_04790)	1060125..1061852	+	1728	WP_220177894.1	terminase family protein	-
FGI21_RS04790 (FGI21_04795)	1061852..1062904	+	1053	WP_220177895.1	phage portal protein	-
FGI21_RS04795 (FGI21_04800)	1063351..1063761	-	411	WP_220177896.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
FGI21_RS04800 (FGI21_04805)	1063789..1063968	-	180	WP_012764944.1	type II toxin-antitoxin system HicA family toxin	Toxin
FGI21_RS04805 (FGI21_04810)	1064297..1064875	+	579	WP_210194408.1	sce7726 family protein	-
FGI21_RS04810 (FGI21_04815)	1064835..1065935	-	1101	WP_220177897.1	beta family protein	-
FGI21_RS04815 (FGI21_04820)	1066153..1068558	-	2406	WP_258570085.1	replication endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	nueA / msbA	984631..1121396	136765

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6530.61 Da
• Isoelectric Point: 10.7838

>T127055 WP_012764944.1 NZ_CP040817:c1063968-1063789 [Dickeya zeae]
MDSRTLIAEIKADGWELVRVNGSHHHFTHPTKPGLVTIPHPKKDLPIGTVKSIRKQAGI

Nucleotide

• Length: 180 bp

>T127055 NZ_CP040817:c1063968-1063789 [Dickeya zeae]
ATGGACAGCAGGACGTTAATAGCAGAAATCAAGGCTGATGGGTGGGAGTTAGTAAGGGTTAACGGCAGTCATCATCATTT
TACCCACCCAACAAAACCGGGATTAGTCACCATCCCACACCCGAAGAAGGATTTACCGATAGGTACGGTAAAAAGCATCA
GGAAACAAGCGGGGATATGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14499.48 Da
• Isoelectric Point: 4.3494

>AT127055 WP_220177896.1 NZ_CP040817:c1063761-1063351 [Dickeya zeae]
MFYPIAIEAGDDTHAYGVTVPDLPGCFSAGDTLDEAIANAKEAITGHIELLIEMGQDIPAVSSVGTLAKSPEYAGYTWAV
VDIDVTRLMGGAEKINVTLPKSLIDRIDRCVASNPEFKSRSGFLAQAALERISSIR

Nucleotide

• Length: 411 bp

>AT127055 NZ_CP040817:c1063761-1063351 [Dickeya zeae]
ATGTTTTATCCAATTGCAATTGAAGCAGGCGACGATACTCATGCATACGGCGTGACAGTGCCAGATCTGCCGGGCTGTTT
CTCCGCAGGTGATACGCTTGATGAGGCCATCGCCAATGCCAAAGAAGCGATCACCGGCCATATCGAGCTGCTGATCGAGA
TGGGTCAGGATATTCCTGCTGTGTCATCCGTCGGAACGCTGGCGAAGTCACCAGAGTACGCCGGTTACACCTGGGCTGTT
GTTGATATCGATGTCACCCGGCTGATGGGTGGGGCAGAAAAAATTAACGTCACGTTGCCGAAATCTCTGATTGACCGTAT
TGACCGCTGCGTAGCGAGTAACCCGGAATTTAAGAGCCGCTCAGGGTTCCTTGCTCAGGCAGCATTAGAGCGGATTTCAT
CCATTCGCTAA

Structures

Toxin

• AlphaFold DB: A0A2K8W4W9