Detailed information of TA system TA127045

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4172173..4172957
• Replicon: chromosome
• Accession: NZ_CP040815
• Organism: Dickeya zeae strain A5272

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: FGI00_RS18410
• Protein ID: WP_248588439.1
• Coordinates: 4172173..4172667 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: FGI00_RS18415
• Protein ID: WP_248588440.1
• Coordinates: 4172664..4172957 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGI00_RS18380 (FGI00_18595)	4168085..4168447	+	363	WP_023638867.1	divalent cation tolerance protein CutA	-
FGI00_RS18385 (FGI00_18600)	4168423..4170171	+	1749	WP_248588436.1	protein-disulfide reductase DsbD	-
FGI00_RS18390 (FGI00_18605)	4170264..4170839	+	576	WP_248589836.1	transcriptional regulator	-
FGI00_RS18400 (FGI00_18615)	4171508..4171726	+	219	WP_248588437.1	type II toxin-antitoxin system antitoxin CcdA	-
FGI00_RS18405 (FGI00_18620)	4171729..4172034	+	306	WP_248588438.1	type II toxin-antitoxin system toxin CcdB	-
FGI00_RS18410 (FGI00_18625)	4172173..4172667	-	495	WP_248588439.1	GNAT family N-acetyltransferase	Toxin
FGI00_RS18415 (FGI00_18630)	4172664..4172957	-	294	WP_248588440.1	DUF1778 domain-containing protein	Antitoxin
FGI00_RS18420 (FGI00_18635)	4173154..4173840	-	687	WP_248588441.1	DUF5710 domain-containing protein	-
FGI00_RS18425 (FGI00_18640)	4174298..4174798	-	501	WP_248588442.1	serine acetyltransferase	-
FGI00_RS18430 (FGI00_18645)	4174828..4176222	-	1395	WP_248588443.1	DUF4832 domain-containing protein	-
FGI00_RS18435 (FGI00_18650)	4176260..4176667	-	408	WP_226099917.1	hypothetical protein	-
FGI00_RS18440 (FGI00_18655)	4176714..4177817	-	1104	WP_012883267.1	glycosyltransferase family 4 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (4-95)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17734.69 Da
• Isoelectric Point: 7.7026

>T127045 WP_248588439.1 NZ_CP040815:c4172667-4172173 [Dickeya zeae]
MISAPEPLRAEHVLSSFCCGVESMDNWLKQRAMKNQVSGASRTFVCCDDAKVLAYYSLASSAVMTNAAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGQGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPIDPMMLMVTLGDLVG
SVSI

Nucleotide

• Length: 495 bp

>T127045 NZ_CP040815:c4172667-4172173 [Dickeya zeae]
ATGATTTCTGCTCCTGAGCCACTCCGCGCCGAACATGTACTTTCCTCATTCTGTTGCGGTGTGGAGTCTATGGACAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTATCTGGCGCATCACGGACTTTCGTTTGTTGTGATGACGCTAAGGTACTGG
CGTATTACTCTTTGGCCTCAAGTGCCGTGATGACAAATGCTGCACCCGGACGCTTTCGCCGTAACATGCCTGATCCGATC
CCGGTGGTGGTGTTGGGGCGTCTGGCGGTAGATAAATCATTGCATGGTCAGGGCGTTGGCCGGGCCTTGGTAAGGGATGC
CGGGCTACGGGTGATTCAGGTGGCCGAGACTATCGGTATTCGCGGAATGTTGGTTCATGCGCTGTCTGATGAAGCGCGTG
ATTTTTACCTGCGGGTGGGATTTGAGCCTTCACCAATAGACCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTTGGG
AGTGTATCAATTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10875.40 Da
• Isoelectric Point: 9.8590

>AT127045 WP_248588440.1 NZ_CP040815:c4172957-4172664 [Dickeya zeae]
MPATNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLTRLDQAPSPN
AALRKTMQTPAPWEQKT

Nucleotide

• Length: 294 bp

>AT127045 NZ_CP040815:c4172957-4172664 [Dickeya zeae]
ATGCCAGCGACGAACAGCGTTTCAGTTAAACGCGAAACATTGAATTTACGCATCAAGCCCGCCGAACGCGATCTGATAGA
TCGCGCTGCGAAGGCCAGAGGAAAAAATCGTACTGATTTTGTCCTCGAAGCTGCTCGAGCTGCCGCAGAGGAAGCGTTGA
TCGATCAGCGCATCATCATGGCAGATCCCGATGCTTATCAGGAATTCCTCACCCGTTTGGATCAGGCTCCTTCACCAAAT
GCTGCGCTGCGAAAAACCATGCAAACGCCTGCACCGTGGGAACAGAAAACATGA