Detailed information of TA system TA127043

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4098319..4099022
• Replicon: chromosome
• Accession: NZ_CP040815
• Organism: Dickeya zeae strain A5272

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: FGI00_RS18055
• Protein ID: WP_121575864.1
• Coordinates: 4098319..4098660 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: FGI00_RS18060
• Protein ID: WP_186957569.1
• Coordinates: 4098681..4099022 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGI00_RS18035 (FGI00_18240)	4093737..4093952	+	216	WP_001144069.1	30S ribosomal protein S21	-
FGI00_RS18040 (FGI00_18245)	4094081..4095835	+	1755	WP_168364913.1	DNA primase	-
FGI00_RS18045 (FGI00_18250)	4095992..4097827	+	1836	WP_016943764.1	RNA polymerase sigma factor RpoD	-
FGI00_RS18055 (FGI00_18260)	4098319..4098660	-	342	WP_121575864.1	TA system toxin CbtA family protein	Toxin
FGI00_RS18060 (FGI00_18265)	4098681..4099022	-	342	WP_186957569.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FGI00_RS18065 (FGI00_18270)	4099033..4099563	-	531	WP_248588399.1	DNA repair protein RadC	-
FGI00_RS18070 (FGI00_18275)	4099576..4100019	-	444	WP_248588400.1	antirestriction protein	-
FGI00_RS18075 (FGI00_18280)	4100050..4100871	-	822	WP_248588401.1	DUF932 domain-containing protein	-
FGI00_RS18080 (FGI00_18285)	4100970..4101200	-	231	WP_119834391.1	DUF905 domain-containing protein	-
FGI00_RS18085 (FGI00_18290)	4101272..4101721	-	450	WP_071199563.1	IrmA family protein	-
FGI00_RS18090 (FGI00_18295)	4101718..4102170	-	453	WP_100168667.1	hypothetical protein	-
FGI00_RS18095 (FGI00_18300)	4102185..4102678	-	494	Protein_3531	hypothetical protein	-
FGI00_RS18100 (FGI00_18305)	4102691..4103811	+	1121	WP_248587941.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4079154..4117091	37937

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12850.81 Da
• Isoelectric Point: 9.6542

>T127043 WP_121575864.1 NZ_CP040815:c4098660-4098319 [Dickeya zeae]
MKTLPATISRAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAEITLADAVNFLVDKYGLVRIDRRGF
NWQEQSPYLRAVDILRARQATGLLRRSRNNAVS

Nucleotide

• Length: 342 bp

>T127043 NZ_CP040815:c4098660-4098319 [Dickeya zeae]
ATGAAAACTCTACCTGCTACAATTTCGCGGGCGGCGAAGCCCTGTCTGTCGCCCGTTGCAGTCTGGCAAATGTTACTGAC
ACGCCTACTGGAACAGCACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACAGTTATTAAGGAACATATTG
ATGCCGAGATTACCCTGGCCGATGCAGTCAACTTCCTGGTGGATAAGTACGGACTGGTTCGTATCGATCGCAGGGGGTTT
AACTGGCAGGAACAATCGCCTTATCTTCGGGCTGTAGATATTCTGCGAGCACGACAAGCAACTGGCTTGCTGCGACGGAG
CCGTAATAATGCTGTATCGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12794.35 Da
• Isoelectric Point: 6.0591

>AT127043 WP_186957569.1 NZ_CP040815:c4099022-4098681 [Dickeya zeae]
MKYTDSENDSFLNWGLNRNVTPCFGARLVQEGNRLHYLADRASITGTFSNAESLKLDEVFPRFISQMESMLTTGEMNPRH
AHCVTLYHNGFTCEADTLGSCGYVYIAVYPTQR

Nucleotide

• Length: 342 bp

>AT127043 NZ_CP040815:c4099022-4098681 [Dickeya zeae]
ATGAAATATACTGACTCAGAGAACGATTCATTTCTCAACTGGGGTCTGAATCGTAATGTGACGCCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGACTTCACTATCTGGCTGACCGGGCCAGCATTACCGGTACATTCAGTAACGCAGAATCTT
TGAAGTTGGATGAAGTATTCCCACGTTTTATCAGCCAGATGGAATCGATGTTGACCACTGGTGAAATGAATCCTCGCCAT
GCCCACTGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACACTTGGCAGTTGCGGCTACGTATACATCGC
CGTTTATCCCACTCAACGTTAA