Detailed information of TA system TA127012

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3718576..3719126
• Replicon: chromosome
• Accession: NZ_CP040805
• Organism: Escherichia fergusonii strain EFCF056

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: D8Z79_RS17975
• Protein ID: WP_125400801.1
• Coordinates: 3718576..3718890 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: D8Z79_RS17980
• Protein ID: WP_000125566.1
• Coordinates: 3718893..3719126 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8Z79_RS17950	3714245..3715531	+	1287	WP_000800490.1	peptidylprolyl isomerase SurA	-
D8Z79_RS17955	3715531..3716520	+	990	WP_046076786.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
D8Z79_RS17960	3716517..3717338	+	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
D8Z79_RS17965	3717341..3717718	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
D8Z79_RS17970	3717725..3718579	+	855	WP_125400802.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
D8Z79_RS17975	3718576..3718890	-	315	WP_125400801.1	CcdB family protein	Toxin
D8Z79_RS17980	3718893..3719126	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
D8Z79_RS17985	3719212..3719691	-	480	WP_000624375.1	type 3 dihydrofolate reductase	-
D8Z79_RS17990	3719884..3721746	-	1863	WP_125400800.1	glutathione-regulated potassium-efflux system protein KefC	-
D8Z79_RS17995	3721739..3722269	-	531	WP_000600701.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
D8Z79_RS18000	3722377..3723708	-	1332	WP_125400799.1	MFS transporter	-
D8Z79_RS18005	3723767..3724054	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11733.68 Da
• Isoelectric Point: 7.3587

>T127012 WP_125400801.1 NZ_CP040805:c3718890-3718576 [Escherichia fergusonii]
MQFTVYRSRSRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSHIHPPERLNPILLLVDGKEYVLMTHETATVPVNTLG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T127012 NZ_CP040805:c3718890-3718576 [Escherichia fergusonii]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCATATTCACCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACACGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACTTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT127012 WP_000125566.1 NZ_CP040805:c3719126-3718893 [Escherichia fergusonii]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT127012 NZ_CP040805:c3719126-3718893 [Escherichia fergusonii]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9