Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3470476..3471059 Replicon chromosome
Accession NZ_CP040805
Organism Escherichia fergusonii strain EFCF056

Toxin (Protein)


Gene name mazF Uniprot ID B7LWP2
Locus tag D8Z79_RS16835 Protein ID WP_000254732.1
Coordinates 3470476..3470811 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag D8Z79_RS16840 Protein ID WP_000581937.1
Coordinates 3470811..3471059 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8Z79_RS16820 3466363..3467661 - 1299 WP_000036723.1 phosphopyruvate hydratase -
D8Z79_RS16825 3467749..3469386 - 1638 WP_000210873.1 CTP synthase (glutamine hydrolyzing) -
D8Z79_RS16830 3469614..3470405 - 792 WP_001071668.1 nucleoside triphosphate pyrophosphohydrolase -
D8Z79_RS16835 3470476..3470811 - 336 WP_000254732.1 endoribonuclease MazF Toxin
D8Z79_RS16840 3470811..3471059 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
D8Z79_RS16845 3471137..3473371 - 2235 WP_000226845.1 GTP diphosphokinase -
D8Z79_RS16850 3473422..3474720 - 1299 WP_000046872.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12156.08 Da        Isoelectric Point: 7.7128

>T127011 WP_000254732.1 NZ_CP040805:c3470811-3470476 [Escherichia fergusonii]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSDQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T127011 NZ_CP040805:c3470811-3470476 [Escherichia fergusonii]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGATCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTGAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT127011 WP_000581937.1 NZ_CP040805:c3471059-3470811 [Escherichia fergusonii]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT127011 NZ_CP040805:c3471059-3470811 [Escherichia fergusonii]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCTCCTGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB B7LWP2

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References