Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1773924..1774514 Replicon chromosome
Accession NZ_CP040805
Organism Escherichia fergusonii strain EFCF056

Toxin (Protein)


Gene name pemK Uniprot ID V0VE57
Locus tag D8Z79_RS08685 Protein ID WP_000373724.1
Coordinates 1773924..1774256 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag D8Z79_RS08690 Protein ID WP_000288812.1
Coordinates 1774257..1774514 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8Z79_RS08660 1770236..1771222 + 987 Protein_1672 integrase arm-type DNA-binding domain-containing protein -
D8Z79_RS08670 1772191..1773075 - 885 WP_001553122.1 integrase domain-containing protein -
D8Z79_RS26065 1773669..1773917 + 249 WP_176550764.1 hypothetical protein -
D8Z79_RS08685 1773924..1774256 - 333 WP_000373724.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
D8Z79_RS08690 1774257..1774514 - 258 WP_000288812.1 antitoxin Antitoxin
D8Z79_RS08695 1774612..1774803 + 192 WP_125400862.1 hypothetical protein -
D8Z79_RS08700 1774862..1775068 - 207 WP_125400861.1 helix-turn-helix domain-containing protein -
D8Z79_RS08705 1775065..1775520 - 456 WP_125400884.1 hypothetical protein -
D8Z79_RS08710 1776187..1778229 - 2043 WP_125400860.1 hypothetical protein -
D8Z79_RS08715 1778234..1778506 - 273 WP_139535880.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1773924..1794545 20621


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11724.57 Da        Isoelectric Point: 10.5834

>T127001 WP_000373724.1 NZ_CP040805:c1774256-1773924 [Escherichia fergusonii]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARSGKRLERIPDAVVNEVLARLEAILS

Download         Length: 333 bp

>T127001 NZ_CP040805:c1774256-1773924 [Escherichia fergusonii]
ATGGACAGAGGGGAAATCTGGCTGGTTTCACTGGATCCGATTGCAGGTCACGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCTGTTGTTGTTCCCGTTACCAGCGGTGGAAACTTCGCCCGTG
CAGCCGGTTTTACCGTCTCACTGGACGGTGCGGGAACAAAAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGGAGCCCGGAGCGGTAAGCGACTGGAACGTATACCCGATGCTGTCGTGAATGAAGTGCTGGCCCGACTGGAGGC
CATTCTGAGCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT127001 WP_000288812.1 NZ_CP040805:c1774514-1774257 [Escherichia fergusonii]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT127001 NZ_CP040805:c1774514-1774257 [Escherichia fergusonii]
ATGTATACAACTCGCCTGAAAAAGGTTGGCGGATCCATCATGCTGGCGGTTCCCCCCGCCGTGCTAAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGACCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCAGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGTAAGGAGATCCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VE57

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References