Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 941763..942417 Replicon chromosome
Accession NZ_CP040805
Organism Escherichia fergusonii strain EFCF056

Toxin (Protein)


Gene name cptA Uniprot ID F0JPE9
Locus tag D8Z79_RS04555 Protein ID WP_000244774.1
Coordinates 942010..942417 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag D8Z79_RS04550 Protein ID WP_000354046.1
Coordinates 941763..942029 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8Z79_RS04525 936901..937794 + 894 WP_046076899.1 transporter -
D8Z79_RS04530 937842..939275 - 1434 WP_139535875.1 6-phospho-beta-glucosidase BglA -
D8Z79_RS04535 939320..939631 + 312 WP_001182952.1 N(4)-acetylcytidine aminohydrolase -
D8Z79_RS04540 939795..940454 + 660 WP_000250283.1 hemolysin III family protein -
D8Z79_RS04545 940531..941511 - 981 WP_125401156.1 tRNA-modifying protein YgfZ -
D8Z79_RS04550 941763..942029 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
D8Z79_RS04555 942010..942417 + 408 WP_000244774.1 protein YgfX Toxin
D8Z79_RS04560 942457..942978 - 522 WP_001055871.1 flavodoxin FldB -
D8Z79_RS04565 943090..943986 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
D8Z79_RS04570 944011..944721 + 711 WP_046076902.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
D8Z79_RS04575 944727..946460 + 1734 WP_125401155.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16078.05 Da        Isoelectric Point: 11.2511

>T126997 WP_000244774.1 NZ_CP040805:942010-942417 [Escherichia fergusonii]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T126997 NZ_CP040805:942010-942417 [Escherichia fergusonii]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGTTAAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCCCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCGCCGTGGATGATTAAAAGCGGCATGATGTTGCGTTTACGTTCTGATAGCGGTAAACGTCA
GCACCTGTGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT126997 WP_000354046.1 NZ_CP040805:941763-942029 [Escherichia fergusonii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT126997 NZ_CP040805:941763-942029 [Escherichia fergusonii]
ATGGACATTAATAACAAAGCCCGCATTCACTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGTGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTCAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L6ZX35

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References