Detailed information of TA system TA126989

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 2644602..2645710
• Replicon: chromosome
• Accession: NZ_CP040801
• Organism: Staphylococcus aureus strain S15

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: FHD66_RS13010
• Protein ID: WP_000233000.1
• Coordinates: 2644602..2645471 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: FHD66_RS13015
• Protein ID: WP_000205227.1
• Coordinates: 2645486..2645710 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FHD66_RS12975	2640095..2640832	-	738	WP_189912527.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
FHD66_RS12980	2640957..2641040	-	84	WP_001814874.1	23S rRNA methyltransferase attenuation leader peptide	-
FHD66_RS12985	2641089..2641172	-	84	Protein_2512	peptide-binding protein	-
FHD66_RS12990	2641582..2642376	-	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
FHD66_RS12995	2642469..2642949	-	481	Protein_2514	GNAT family N-acetyltransferase	-
FHD66_RS13000	2642946..2643854	-	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
FHD66_RS13005	2643887..2644621	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
FHD66_RS13010	2644602..2645471	-	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
FHD66_RS13015	2645486..2645710	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
FHD66_RS13020	2645853..2647415	-	1563	WP_000136908.1	recombinase family protein	-
FHD66_RS13025	2647417..2647833	-	417	WP_000323438.1	recombinase	-
FHD66_RS13030	2647834..2648130	-	297	WP_002360685.1	hypothetical protein	-
FHD66_RS13035	2648642..2649319	-	678	Protein_2522	DNA topoisomerase	-
FHD66_RS13040	2649335..2649937	-	603	WP_001062586.1	recombinase family protein	-
FHD66_RS13045	2649951..2650121	-	171	WP_000713595.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	erm(B) / aph(3')-III / ant(6)-Ia	-	2639959..2652210	12251
	-	flank	IS/Tn	erm(B) / aph(3')-III / ant(6)-Ia	-	2635179..2643854	8675

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T126989 WP_000233000.1 NZ_CP040801:c2645471-2644602 [Staphylococcus aureus]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T126989 NZ_CP040801:c2645471-2644602 [Staphylococcus aureus]
ATGGTAGGTAATGTTATTCAAATAGTAACAGAGAAATTATCATCTTTGCCTTTTATAGAAGGCATCGTATTAGGGGGCTC
ACGTGCAAGAGGCACCCATACAGAAAATTCTGATATAGATATCGGAATCTATTACAATTCAGACTCATTTGACCTGACAG
CGATTAATCAAATTGCTACAGAATTGGATGATAAAAATAGAAACAACCTTGTTGTGCCTCCCGGAGCGTGGGGTGATTGG
ATTAATGGCGGCGGATGGTTATTTATAAATGGGTATCATGTTGACTTGATTTTACGTGATATAAAACGGGTAGAACAAAT
AATCAAAGATACGGAGCAAGGAATTGTTACTGCCAATTATCAGACCGGGCATCCCCATGGTTATATAAGTGCAATGTATC
GTGGAGAATTAGCGATTAGTAAAATACTATATGCTAAGAACGAAAGCTTATGCGAATTAAAAAAGCAGGCAGAAATTTAC
CCTACTGCTCTAAAGAAAAGCTTGATGAACTTTTTTATATTTGAAGCTGAATTCTCTTTAATGTTTGTAAAAGCAAATGC
GGGGGTAGAGGATAAATATTATATTGCAGGCCATGTTTTTCGTATTATTTCATGCTTAAATCAAGTACTATTTGCATGTA
ATAATGCTTATTGCATTAACGAAAAGAAAGCTATAAAACTGCTTGAAACTTTTGAATATAAACCTGAAAAATATGCCGAA
AGAGTTAATCATATTTTTGAAGTACTCGGTCTTTCTCTTTTTGAATGCTACGATATGACCGAGAAACTTTATAAAGAAGT
GAATGAAATTGTATCGGAGATAAATAACTTTTTAAACGAGGAGAGTTCAGATGAAAGAAAACAAATATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT126989 WP_000205227.1 NZ_CP040801:c2645710-2645486 [Staphylococcus aureus]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT126989 NZ_CP040801:c2645710-2645486 [Staphylococcus aureus]
ATGACTACGGCAGAAATGATTAAAGAACTGTGTGAGCAAATGAATATAAGTGTTTCCGAACTTGCTAGACGTATTGGCCA
GACTCCACAGAATTTCAATAAAAAATTACAACGAGAAACAGTAACCTTGGATGAGTTGAAGGCCATTGCTGATGTGCTAG
GTGTCAAGTTTGTGCAGGCATTTATTTTACCAGATGGAGAAGAAATAAAAATATCTAACGAATAA

Structures

Toxin

• AlphaFold DB: R3JIN1