Detailed information of TA system TA126971

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-DUF971
• Location: 1388490..1389095
• Replicon: chromosome
• Accession: NZ_CP040799
• Organism: Xylella fastidiosa subsp. fastidiosa strain Bakersfield-1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: B2I5N3
• Locus tag: FG899_RS06380
• Protein ID: WP_004089252.1
• Coordinates: 1388490..1388786 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: FG899_RS06385
• Protein ID: WP_004089254.1
• Coordinates: 1388790..1389095 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FG899_RS06355	1383655..1384632	+	978	WP_004087353.1	hypothetical protein	-
FG899_RS06360	1384891..1385337	+	447	WP_004087355.1	hypothetical protein	-
FG899_RS06365	1385441..1386685	-	1245	WP_011097983.1	phage tail protein	-
FG899_RS06370	1386689..1387249	-	561	WP_011097869.1	DUF2612 domain-containing protein	-
FG899_RS06375	1387246..1388391	-	1146	WP_011097984.1	baseplate J/gp47 family protein	-
FG899_RS06380	1388490..1388786	+	297	WP_004089252.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FG899_RS06385	1388790..1389095	+	306	WP_004089254.1	putative addiction module antidote protein	Antitoxin
FG899_RS06390	1389106..1389459	-	354	WP_011097986.1	hypothetical protein	-
FG899_RS06395	1389456..1390097	-	642	WP_004090764.1	hypothetical protein	-
FG899_RS06400	1390094..1390924	-	831	WP_004090766.1	hypothetical protein	-
FG899_RS06405	1390921..1391238	-	318	WP_011097873.1	hypothetical protein	-
FG899_RS06410	1391238..1392008	-	771	WP_004090769.1	hypothetical protein	-
FG899_RS06415	1392119..1392346	+	228	WP_004091377.1	hypothetical protein	-
FG899_RS06420	1392330..1392596	+	267	WP_011097988.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1385441..1403740	18299

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (49-95)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10790.57 Da
• Isoelectric Point: 10.0509

>T126971 WP_004089252.1 NZ_CP040799:1388490-1388786 [Xylella fastidiosa subsp. fastidiosa]
MVELIKTSTFDAWINSLRDRKAAARIQARLDRLALGNPGDVKPVGAGISEMRIDHGPGYRIYFMKHGAVLILLLCGGDKS
SQVRDIEQAKALAALWKD

Nucleotide

• Length: 297 bp

>T126971 NZ_CP040799:1388490-1388786 [Xylella fastidiosa subsp. fastidiosa]
ATGGTCGAACTCATCAAAACCAGCACTTTTGATGCTTGGATAAACAGCTTACGTGACCGCAAAGCAGCCGCCAGAATTCA
AGCGCGTCTTGATCGGCTCGCACTTGGCAATCCCGGCGACGTGAAGCCTGTGGGTGCTGGCATCTCAGAAATGAGGATCG
ACCATGGGCCCGGATATCGGATCTATTTTATGAAGCATGGTGCAGTACTGATTCTCTTACTTTGCGGTGGCGATAAATCG
TCACAAGTACGAGATATAGAGCAGGCTAAGGCACTGGCTGCACTGTGGAAGGATTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10983.37 Da
• Isoelectric Point: 5.0941

>AT126971 WP_004089254.1 NZ_CP040799:1388790-1389095 [Xylella fastidiosa subsp. fastidiosa]
MNNETFSRYDTADYLKTEEDIAAYMEAVMEEGGRDNPAFIARALGAVARARNLSQLARDVGMSRQGLDKALSNDGNPSFS
TILKVAKALGLRMSFTPSSMS

Nucleotide

• Length: 306 bp

>AT126971 NZ_CP040799:1388790-1389095 [Xylella fastidiosa subsp. fastidiosa]
ATGAATAATGAGACTTTTAGTCGATACGACACCGCTGACTATCTCAAAACCGAAGAAGATATTGCGGCTTATATGGAAGC
CGTCATGGAAGAAGGTGGTAGGGATAATCCTGCTTTTATTGCACGGGCACTTGGTGCTGTCGCTCGTGCTCGTAATTTAA
GTCAGCTTGCTCGTGACGTTGGCATGTCCAGGCAAGGACTAGACAAGGCACTCTCTAACGACGGCAACCCAAGCTTCTCA
ACCATCTTAAAAGTGGCTAAAGCGCTTGGTTTACGGATGTCATTCACACCTTCCTCTATGAGTTAA

Structures

Toxin

• AlphaFold DB: A0A060HBK9