Detailed information of TA system TA126969

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): FicTA/Fic(toxin)
• Location: 1216333..1217129
• Replicon: chromosome
• Accession: NZ_CP040799
• Organism: Xylella fastidiosa subsp. fastidiosa strain Bakersfield-1

Toxin (Protein)

• Gene name: VbhT
• Uniprot ID: Q87CQ8
• Locus tag: FG899_RS05445
• Protein ID: WP_004088112.1
• Coordinates: 1216333..1216944 (-)
• Length: 204 a.a.

Antitoxin (Protein)

• Gene name: VbhA
• Uniprot ID: B2I551
• Locus tag: FG899_RS05450
• Protein ID: WP_004088114.1
• Coordinates: 1216941..1217129 (-)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FG899_RS05400	1211788..1212675	-	888	WP_012382597.1	YdaU family protein	-
FG899_RS05405	1212672..1213460	-	789	WP_011097897.1	Bro-N domain-containing protein	-
FG899_RS05410	1213457..1214089	-	633	WP_004088094.1	Bro-N domain-containing protein	-
FG899_RS05415	1214086..1214370	-	285	WP_004088096.1	hypothetical protein	-
FG899_RS05420	1214367..1214615	-	249	WP_011097898.1	hypothetical protein	-
FG899_RS05425	1214612..1214800	-	189	WP_004088102.1	hypothetical protein	-
FG899_RS05430	1214828..1215019	-	192	WP_004088104.1	hypothetical protein	-
FG899_RS05435	1215278..1215523	-	246	WP_004088108.1	hypothetical protein	-
FG899_RS05440	1215608..1216282	+	675	WP_012382600.1	helix-turn-helix transcriptional regulator	-
FG899_RS05445	1216333..1216944	-	612	WP_004088112.1	Fic/DOC family protein	Toxin
FG899_RS05450	1216941..1217129	-	189	WP_004088114.1	antitoxin VbhA family protein	Antitoxin
FG899_RS05455	1217521..1218057	+	537	WP_012382601.1	hypothetical protein	-
FG899_RS05460	1218054..1218467	+	414	WP_004088056.1	DUF1566 domain-containing protein	-
FG899_RS05465	1218464..1218865	+	402	WP_004088054.1	hypothetical protein	-
FG899_RS05470	1218862..1219011	+	150	WP_012382602.1	hypothetical protein	-
FG899_RS05475	1219097..1219375	+	279	WP_004088050.1	hypothetical protein	-
FG899_RS05480	1219398..1219589	+	192	WP_012382603.1	hypothetical protein	-
FG899_RS05485	1219610..1219954	+	345	WP_004088346.1	hypothetical protein	-
FG899_RS05490	1219994..1220815	+	822	WP_004088345.1	DUF2303 family protein	-
FG899_RS05495	1220831..1221757	+	927	WP_004088344.1	phage recombination protein Bet	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1203430..1226879	23449

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (55-152)

Antitoxin

• Antitoxin VbhA (10-56)

Sequences

Toxin

Protein

• Length: 204 a.a.
• Molecular weight: 22832.60 Da
• Isoelectric Point: 5.1271

>T126969 WP_004088112.1 NZ_CP040799:c1216944-1216333 [Xylella fastidiosa subsp. fastidiosa]
MKYAGDRGDPYLDSETGVLRNLLGIRDQGGLDKIESTLSFLRTSELRERPVKGKFDLAHLQEIHNRLFQDVYDWAGQIRQ
VEISKGNTMFAQQIAIQSAAQQIFGQLAKERFLCGLDAEEFSKRAGDYLGEINVLHPFREGNGRTQREFIAQLAQRAGYR
IDWGVVSQADMIKASIDAYNGDSSGLASIIREGISDQLFKDNE

Nucleotide

• Length: 612 bp

>T126969 NZ_CP040799:c1216944-1216333 [Xylella fastidiosa subsp. fastidiosa]
ATGAAATACGCAGGGGATCGTGGTGATCCTTACCTGGACAGCGAAACAGGTGTTCTTCGTAATCTCCTTGGAATCAGGGA
TCAGGGGGGGCTCGATAAAATAGAGTCCACCCTTTCTTTTTTGCGAACCAGCGAATTGCGTGAGCGACCCGTAAAAGGCA
AATTTGATCTAGCGCATTTACAGGAAATCCATAATCGTCTTTTCCAGGATGTGTACGACTGGGCGGGTCAAATCCGTCAA
GTTGAAATCTCGAAAGGCAACACGATGTTTGCCCAGCAGATTGCGATTCAGAGTGCGGCGCAGCAGATCTTTGGGCAGCT
TGCTAAAGAGCGATTTTTGTGTGGCCTTGATGCTGAAGAATTCAGCAAACGGGCAGGTGATTATCTTGGTGAAATTAATG
TGCTTCACCCATTCCGCGAGGGAAATGGCAGGACGCAACGGGAATTCATCGCGCAATTGGCTCAGCGTGCAGGTTACCGG
ATCGACTGGGGCGTGGTCAGCCAGGCGGATATGATTAAAGCGTCAATCGACGCTTACAACGGGGATTCAAGTGGATTGGC
TAGTATCATCCGCGAAGGAATATCTGATCAGCTTTTTAAAGATAATGAGTAA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6837.77 Da
• Isoelectric Point: 4.9859

>AT126969 WP_004088114.1 NZ_CP040799:c1217129-1216941 [Xylella fastidiosa subsp. fastidiosa]
MLTDHEKAERRAVVQSALASQRIEGLEPDAQAVVDAECWARGEMTIAAAVDQYKARVRLQIA

Nucleotide

• Length: 189 bp

>AT126969 NZ_CP040799:c1217129-1216941 [Xylella fastidiosa subsp. fastidiosa]
ATGCTGACAGATCATGAGAAAGCTGAACGCCGTGCTGTGGTGCAAAGCGCACTCGCAAGCCAACGCATTGAGGGGTTAGA
GCCGGATGCGCAGGCCGTGGTTGATGCTGAATGCTGGGCGCGTGGTGAAATGACTATCGCTGCCGCCGTGGACCAGTACA
AAGCGCGTGTTCGGCTCCAGATTGCATGA

Structures

Toxin

• AlphaFold DB: Q87CQ8

Antitoxin

• AlphaFold DB: B2I551