Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/ParE-RHH
Location 2334777..2335297 Replicon chromosome
Accession NZ_CP040797
Organism Clavibacter michiganensis subsp. nebraskensis strain A6096

Toxin (Protein)


Gene name parE Uniprot ID M5BDX6
Locus tag FGQ65_RS11300 Protein ID WP_015491236.1
Coordinates 2335007..2335297 (+) Length 97 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A399PXI2
Locus tag FGQ65_RS11295 Protein ID WP_041465310.1
Coordinates 2334777..2335010 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FGQ65_RS11255 (FGQ65_11225) 2329800..2330645 - 846 WP_015491251.1 endonuclease/exonuclease/phosphatase family protein -
FGQ65_RS11260 (FGQ65_11230) 2330713..2331405 - 693 WP_015491250.1 hypothetical protein -
FGQ65_RS11265 (FGQ65_11235) 2331615..2332085 - 471 WP_015491249.1 MarR family transcriptional regulator -
FGQ65_RS11270 (FGQ65_11240) 2332131..2332367 + 237 WP_015491248.1 hypothetical protein -
FGQ65_RS11275 (FGQ65_11245) 2332392..2332712 - 321 WP_237580720.1 hypothetical protein -
FGQ65_RS11280 (FGQ65_11250) 2333066..2334130 - 1065 WP_237580721.1 LD-carboxypeptidase -
FGQ65_RS11285 (FGQ65_11255) 2334138..2334449 + 312 WP_236685126.1 antitoxin VbhA family protein -
FGQ65_RS11290 (FGQ65_11260) 2334465..2334665 + 201 WP_015491238.1 hypothetical protein -
FGQ65_RS11295 (FGQ65_11265) 2334777..2335010 + 234 WP_041465310.1 type II toxin-antitoxin system ParD family antitoxin Antitoxin
FGQ65_RS11300 (FGQ65_11270) 2335007..2335297 + 291 WP_015491236.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FGQ65_RS11305 (FGQ65_11275) 2335337..2335591 - 255 Protein_2222 helix-turn-helix transcriptional regulator -
FGQ65_RS11310 (FGQ65_11285) 2335750..2337348 - 1599 WP_015491235.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-92)

Antitoxin

Antitoxin ParD

(4-72)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11338.63 Da        Isoelectric Point: 6.4832

>T126964 WP_015491236.1 NZ_CP040797:2335007-2335297 [Clavibacter michiganensis subsp. nebraskensis]
VKPHRLTPAATKDLSEIWDYTEERWDADQAETYVREIGAAIRRVAEDPTRGRDCGDIRPGYRRYSIESHTVYYVDRPETV
DVIRILHQRMDPSRHL

Download         Length: 291 bp

>T126964 NZ_CP040797:2335007-2335297 [Clavibacter michiganensis subsp. nebraskensis]
GTGAAGCCGCACCGGCTCACGCCGGCCGCGACGAAGGACCTCTCCGAGATCTGGGACTACACGGAGGAGCGCTGGGACGC
CGATCAGGCTGAGACCTATGTCCGGGAGATCGGCGCCGCGATCAGGCGGGTCGCCGAAGACCCCACGCGCGGCCGTGACT
GCGGCGACATCCGCCCGGGCTACCGGCGGTACAGCATCGAGAGCCACACCGTGTACTACGTGGACCGACCCGAGACGGTC
GACGTGATCCGGATCCTGCATCAGCGGATGGACCCGTCGCGCCATCTGTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8268.18 Da        Isoelectric Point: 4.4000

>AT126964 WP_041465310.1 NZ_CP040797:2334777-2335010 [Clavibacter michiganensis subsp. nebraskensis]
MAQNTSISLDGHFTGFLAREVESGRYRSASEVVRAGLRLLEDQETQLEALRAALVAGEASGEAAPFDLDAFIADKRA

Download         Length: 234 bp

>AT126964 NZ_CP040797:2334777-2335010 [Clavibacter michiganensis subsp. nebraskensis]
ATGGCGCAGAACACCTCGATCAGCCTGGACGGGCACTTCACCGGCTTCCTCGCCCGGGAGGTGGAGTCCGGGCGCTACCG
ATCCGCGAGCGAGGTGGTCCGCGCCGGGCTCCGTCTCCTGGAGGACCAGGAGACGCAGCTCGAAGCGCTGCGTGCGGCGC
TGGTCGCCGGCGAGGCGAGCGGCGAGGCCGCACCGTTCGACCTCGACGCGTTCATCGCGGACAAGCGCGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M5BDX6

Antitoxin

Source ID Structure
AlphaFold DB A0A399PXI2

References