126961

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 248743..249385 Replicon chromosome
Accession NZ_CP040782
Organism Bacillus thuringiensis strain HM-311

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag FHE73_RS01435 Protein ID WP_000635965.1
Coordinates 249035..249385 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag FHE73_RS01430 Protein ID WP_000004570.1
Coordinates 248743..249030 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FHE73_RS01405 244060..245022 + 963 WP_000961160.1 UV DNA damage repair endonuclease UvsE -
FHE73_RS01410 245015..245587 - 573 WP_000908521.1 rhomboid family intramembrane serine protease -
FHE73_RS01415 245681..246040 + 360 WP_000583417.1 holo-ACP synthase -
FHE73_RS01420 246197..247147 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
FHE73_RS01425 247265..248434 + 1170 WP_000390613.1 alanine racemase -
FHE73_RS01430 248743..249030 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
FHE73_RS01435 249035..249385 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
FHE73_RS01440 249453..251621 + 2169 WP_000426236.1 RNA-binding transcriptional accessory protein -
FHE73_RS01445 251679..251795 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
FHE73_RS01450 251991..252449 + 459 WP_061883709.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T126961 WP_000635965.1 NZ_CP040782:249035-249385 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T126961 NZ_CP040782:249035-249385 [Bacillus thuringiensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
GCAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT126961 WP_000004570.1 NZ_CP040782:248743-249030 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT126961 NZ_CP040782:248743-249030 [Bacillus thuringiensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References